The potential of the culturable bacterial community from an Alpine coniferous forest site for the degradation of organic polymers and pollutants at low (5 °C) and moderate (20 °C) temperatures was evaluated. The majority of the 68 strains belonged to the phylum Proteobacteria (77%). Other strains were related to Bacteroidetes (12%), Alphaproteobacteria (4%), Actinobacteria (3%), and Firmicutes (3%). The strains were grouped into 42 different OTUs. The highest bacterial diversity was found within the phylum Bacteroidetes. All strains, except one, could grow at temperatures from 5 to 25 °C. The production of enzyme activities involved in the degradation of organic polymers present in plant litter (carboxymethyl cellulose, microgranular cellulose, xylan, polygalacturonic acid) was almost comparable at 5 °C (68%) and 20 °C (63%). Utilizers of lignin compounds (lignosulfonic acid, lignin alkali) as sole carbon source were found to a higher extent at 20 °C (57%) than at 5 °C (24%), but the relative fractions among positively tested strains utilizing these compounds were almost identical at the two temperatures. Similar results were noted for utilizers of organic pollutants (n-hexadecane, diesel oil, phenol, glyphosate) as sole carbon source. More than two-thirds showed constitutively expressed catechol-1,2-dioxygenase activity both at 5 °C (74%) and 20 °C (66%). Complete phenol (2.5 mmol/L) degradation by strain Paraburkholderia aromaticivorans AR20-38 was demonstrated at 0–30 °C, amounts up to 7.5 mmol/L phenol were fully degraded at 10–30 °C. These results are useful to better understand the effect of changing temperatures on microorganisms involved in litter degradation and nutrient turnover in Alpine forest soils.

评估了来自高山针叶林地的可培养细菌群落在低 (5 °C) 和中等 (20 °C) 温度下降解有机聚合物和污染物的潜力。68 个菌株中的大多数属于变形菌门 (77%)。其他菌株与拟杆菌 (12%)、Alphaproteobacteria (4%)、放线菌 (3%) 和厚壁菌门 (3%) 相关。这些菌株被分为 42 个不同的 OTU。在拟杆菌门中发现了最高的细菌多样性。除一种外，所有菌株都可以在 5 至 25 °C 的温度下生长。参与降解植物凋落物中的有机聚合物（羧甲基纤维素、微粒纤维素、木聚糖、聚半乳糖醛酸）的酶活性的产生在 5 °C (68%) 和 20 °C (63%) 下几乎相当。使用木质素化合物（木质素磺酸、木质素碱）作为唯一碳源的利用者在 20 °C (57%) 下的程度高于 5 °C (24%)，但使用这些化合物的阳性测试菌株之间的相对比例在两个温度下几乎相同。对于将有机污染物（正十六烷、柴油、苯酚、草甘膦）作为唯一碳源的利用者，也注意到了类似的结果。超过三分之二在 5 °C (74%) 和 20 °C (66%) 下均表现出组成型表达的儿茶酚-1,2-双加氧酶活性。通过应变完全降解苯酚 (2.5 mmol/L) 对于将有机污染物（正十六烷、柴油、苯酚、草甘膦）作为唯一碳源的利用者，也注意到了类似的结果。超过三分之二在 5 °C (74%) 和 20 °C (66%) 下均表现出组成型表达的儿茶酚-1,2-双加氧酶活性。通过应变完全降解苯酚 (2.5 mmol/L) 对于将有机污染物（正十六烷、柴油、苯酚、草甘膦）作为唯一碳源的利用者，也注意到了类似的结果。超过三分之二在 5 °C (74%) 和 20 °C (66%) 下均表现出组成型表达的儿茶酚-1,2-双加氧酶活性。通过应变完全降解苯酚 (2.5 mmol/L)帕拉伯克霍尔德氏菌AR20-38 在 0–30 °C 下被证明，高达 7.5 mmol/L 的苯酚在 10–30 °C 下完全降解。这些结果有助于更好地了解温度变化对高山森林土壤中参与凋落物降解和养分转换的微生物的影响。