In this work, we propose to evaluate the effect of agriculture intensification under greenhouses on the biochemical and transcriptomic responses of the earthworms Eisenia andrei. This work was conducted on two sites in Téboulba and Sahline (Monastir governorate) and a control site in an experimental plot that is undergoing organic farming. For this purpose, the earthworms Eisenia andrei were exposed to the soils during 7 and 14 days. The physicochemical properties of the soils were analyzed. The biochemical biomarkers of metallothioneins (MTs) and malondialdehyde (MDA) accumulations were also assessed. Moreover, the gene expression level of the MTs was analyzed. The results of our study revealed a significant trace element accumulation accompanied by a high level of MDA and MT proteins. Moreover, a significant expression of the MT gene was observed in earthworms exposed to the soils from Sahline and Téboulba. Hence, this work reveals that intensive agriculture can affect the biological responses of earthworms and consequently, the soil’s biofertility.

在这项工作中，我们建议评估温室农业集约化对on Eisenia andrei的生化和转录组学响应的影响。这项工作是在Téboulba和Sahline（莫纳斯提尔省）的两个地点以及一个进行有机耕作的试验区的一个控制地点进行的。为此，EEisenia andrei在7和14天内暴露于土壤中。分析了土壤的理化性质。还评估了金属硫蛋白（MTs）和丙二醛（MDA）积累的生化生物标志物。此外，分析了MT的基因表达水平。我们的研究结果表明，大量的痕量元素伴有高水平的MDA和MT蛋白。此外，在暴露于Sahline和Téboulba的土壤的earth中观察到MT基因的显着表达。因此，这项工作表明，集约化农业会影响worm的生物反应，进而影响土壤的生物肥力。