Abstract—

Gene electrotransfer is an effective and promising gene delivery technique in clinical applications, such as DNA vaccination and gene therapy. An improved gene therapy protocol depends on the the proper establishment of the gene transfer method. Electroporation has been widely employed in in vitro and in vivo protocols, and increaing its transfection efficiency has been the field of research. In order to achieve the the maximal introduction of plasmid DNA into cells with optimal cell viability, electro transfection conditions for every single cell type should be determined individually. In this work, the effect of cell passage time on the electrotransfection efficiency of CHO cells is determined. The selected cell passage times of 24 and 48 h prior to the electroporation are considered for the analysis. It is shown that electrotransfection efficiency with all plasmid concentrations significantly differs when comparing 24 and 48 h cell passage times. However, only slight change in the cell viability is observed at 24 and 48 h of cell passage times.

中文翻译：

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细胞传代时间对电转染效率的影响

摘要—

基因电转移是在临床应用中有效且有前途的基因传递技术，例如DNA疫苗接种和基因治疗。改进的基因治疗方案取决于基因转移方法的正确建立。电穿孔已在体外和体内方案中广泛使用，并且提高其转染效率已成为研究领域。为了以最佳的细胞生存能力最大程度地将质粒DNA引入细胞，应分别确定每种细胞类型的电转染条件。在这项工作中，确定了细胞通过时间对CHO细胞电转染效率的影响。考虑在电穿孔之前选择的24和48小时的细胞通过时间进行分析。结果表明，当比较24和48小时的细胞传代时间时，所有质粒浓度下的电转染效率都显着不同。但是，在细胞传代时间的24和48小时，仅观察到细胞活力的轻微变化。