Fascioliasis is an important neglected tropical disease that causes severe injury to the bile ducts and liver. Therefore, a rapid and accurate method for detection of Fasciola hepatica infection plays a vital role in early treatment. Currently, the diagnosis of fascioliasis is mainly conducted via serological tests using the excretory/secretory (E/S) products, which might cross-react with antigens from other helminth parasitic diseases. Hence, the development of serodiagnosis test using recombinant antigens may contribute to differentiate fascioliasis from other helminth infections. In the past 20 years, many attempts have been made to exert different F. hepatica recombinant antigens to obtain a well-established standard assay with high accuracy. In this review, we address recent studies that refer to the development of serodiagnosis tests for diagnosis of human fascioliasis based on the candidate recombinant antigens produced by different approaches. Meanwhile, in the present review, some main factors have been highlighted to improve the accuracy of diagnostic tests such as the effect of refolding methods to recover antigens’ tertiary structure as well as applying a mixture of recombinant antigens with the highest sensitivity and specificity to improve the accuracy of diagnostic tests.

片形吸虫病是一种重要的被忽视的热带疾病，可对胆管和肝脏造成严重损伤。因此，一种快速准确的肝片吸菌感染检测方法在早期治疗中起着至关重要的作用。目前，片形吸虫病的诊断主要通过使用排泄/分泌 (E/S) 产品的血清学检测进行，这些产品可能与其他蠕虫寄生虫病的抗原发生交叉反应。因此，使用重组抗原的血清学诊断测试的发展可能有助于区分片吸虫病与其他蠕虫感染。在过去的 20 年中，已经进行了许多尝试以发挥不同的肝 F.重组抗原，以获得高度准确的完善的标准测定。在这篇综述中，我们讨论了最近的研究，这些研究涉及基于不同方法产生的候选重组抗原开发用于诊断人片吸虫病的血清诊断测试。同时，在本综述中，一些主要因素已被强调以提高诊断测试的准确性，例如重折叠方法对恢复抗原三级结构的影响，以及应用具有最高灵敏度和特异性的重组抗原混合物来提高诊断测试的准确性。诊断测试的准确性。