The current study deals with the investigation of the antioxidant, anti-inflammatory and immunomodulatory properties of the essential oil from Datura stramonium leaves (D. oil). The GC-MS analysis showed that the dominant compounds present in the D. oil were neophytadiene (Phytol acetate) (10.76%), β-damascenone (9.67%), and β- eudesmol (7.2%). D. oil exhibited in vitro scavenging potential of free radicals by DPPH and ABTS assays (IC₅₀ values 71.35 ±1.06 μg/ml and 61.01 ± 1.07 μg/ml, respectively). We found that D. oil decreased the nitric oxide production in LPS-stimulated J774A.1 cells by 52.43% without affecting their cell viability. D. oil was found to stimulate the proliferation of human peripheral blood mononuclear cells (PBMC) and, also enhanced the secretion of IL-2, IFN-γ and TNF-α. Furthermore, D. oil treatment of PBMC induced the expression of CD3, CD8, and CD56 and intracellular granulysin levels in the immune cells. The treatment of human lymphocytes by D. oil enhanced their ability to kill colon cancer cells HCT-116 (51.09 ± 7.5%) and SW620 (48.57 ± 8.08%) at 20:1 (effector: target ratio). Moreover, these activated lymphocytes cause target cell death by reactive oxygen species and by damaging mitochondrial membrane potential of these cells. Taken together, the current findings showed D. oil as immunotherapeutic agent which can be used for colon cancer treatment.

目前的研究涉及对来自曼陀罗叶 (D. oil)的精油的抗氧化、抗炎和免疫调节特性的研究。GC-MS 分析表明，D.油中存在的主要化合物是新植二烯（醋酸植醇酯）（10.76%）、β-大马酮（9.67%）和β-丁香酚（7.2%）。D. 油通过 DPPH 和 ABTS 测定（IC ₅₀值分别为 71.35 ±1.06 μg/ml 和 61.01 ± 1.07 μg/ml）。我们发现 D. oil 将 LPS 刺激的 J774A.1 细胞中的一氧化氮产量降低了 52.43%，而不会影响它们的细胞活力。D. 油被发现可以刺激人外周血单核细胞 (PBMC) 的增殖，并且还可以增强 IL-2、IFN-γ 和 TNF-α 的分泌。此外，PBMC 的 D. oil 处理诱导了免疫细胞中 CD3、CD8 和 CD56 的表达以及细胞内颗粒溶素水平。用 D. oil 处理人淋巴细胞增强了它们以 20:1（效应子：靶标比率）杀死结肠癌细胞 HCT-116 (51.09 ± 7.5%) 和 SW620 (48.57 ± 8.08%) 的能力。此外，这些活化的淋巴细胞通过活性氧和破坏这些细胞的线粒体膜电位导致靶细胞死亡。综合起来，