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Sphingomonas horti sp. nov., a novel bacterial species isolated from soil of a tomato garden
Archives of Microbiology ( IF 2.3 ) Pub Date : 2020-09-24 , DOI: 10.1007/s00203-020-02056-x
Shahina Akter 1 , Sun-Young Lee 2 , Sung-Kwon Moon 2 , Changsun Choi 2 , Sri Renukadevi Balusamy 3 , Muhammad Zubair Siddiqi 4 , Md Ashrafudoulla 5 , Md Amdadul Huq 2
Affiliation  

A novel bacterial strain, designated MAH-20T, was isolated from a soil sample of a tomato garden. Cells of strain MAH-20T were Gram-stain negative, aerobic, motile, and rod-shaped. The colonies were light brown colored, smooth, spherical, and 0.2-0.7 mm in diameter when grown on Luria-Bertani agar for 2 days. Strain MAH-20T grows at 15-40 °C (optimum growth temperature 30-32 °C), at pH 5.0-10.0 (optimum growth pH 7.0) and at 0-2.0% NaCl. The strain showed positive activity for both oxidase and catalase tests. Cells were able to hydrolyze starch, DNA, urea, gelatin, L-arginine, and Tween 20. According to the 16S rRNA gene sequence similarity, the strain MAH-20T was identified as a new member of the genus Sphingomonas and had the close sequence similarity with Sphingomonas changbaiensis V2M44T (98.9%) and Sphingomonas tabacisoli X1-8T (98.1%). The genomic ANI value between strain MAH-20T and S. changbaiensis NBRC 104936T was 84.4%. The novel strain MAH-20T has a draft genome size of 3,350,026 bp (25 contigs), annotated with 3210 protein-coding genes, 46 tRNA, and 3 rRNA genes. The genomic DNA G + C content of isolate was 67.3 mol%, the predominant quinone was ubiquinone 10 and the major fatty acids were C16:0, C17:1 ω6c and summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c). On the basis of DNA-DNA hybridization results, phenotypic, genotypic, and chemotaxonomic data, the isolated strain MAH-20T represents a novel species, for which the name Sphingomonas horti sp. nov. is proposed, with MAH-20T as the type strain (= KACC 19746T = CGMCC1.13658T).

中文翻译:

鞘氨醇单胞菌 11 月,一种从番茄园土壤中分离的新型细菌

从番茄园的土壤样本中分离出一种名为 MAH-20T 的新型细菌菌株。MAH-20T 菌株的细胞为革兰氏染色阴性、需氧、活动和杆状。当在 Luria-Bertani 琼脂上生长 2 天时,菌落呈浅棕色、光滑、球形,直径为 0.2-0.7 毫米。菌株 MAH-20T 在 15-40 °C(最佳生长温度 30-32 °C)、pH 5.0-10.0(最佳生长 pH 7.0)和 0-2.0% NaCl 下生长。该菌株在氧化酶和过氧化氢酶试验中均显示出阳性活性。细胞能够水解淀粉、DNA、尿素、明胶、L-精氨酸和吐温20。根据16S rRNA基因序列相似性,菌株MAH-20T被鉴定为鞘氨醇单胞菌属的新成员,具有相近的序列与Sphingomonas changbaiensis V2M44T (98.9%) 和Sphingomonas tabacisoli X1-8T (98.1%) 的相似性。MAH-20T菌株与长白链球菌NBRC 104936T的基因组ANI值为84.4%。新菌株 MAH-20T 的基因组草图大小为 3,350,026 bp(25 个重叠群),注释有 3210 个蛋白质编码基因、46 个 tRNA 和 3 个 rRNA 基因。分离株的基因组DNA G+C含量为67.3 mol%,主要的醌为泛醌10,主要脂肪酸为C16:0、C17:1 ω6c和总特征8(包括C18:1 ω7c和/或C18:1 ω6c)。根据 DNA-DNA 杂交结果、表型、基因型和化学分类数据,分离的菌株 MAH-20T 代表了一个新物种,其名称为 Sphinomonas horti sp。十一月 提出,以 MAH-20T 作为类型菌株(= KACC 19746T = CGMCC1.13658T)。350,026 bp(25 个重叠群),用 3210 个蛋白质编码基因、46 个 tRNA 和 3 个 rRNA 基因注释。分离株的基因组DNA G+C含量为67.3 mol%,主要的醌为泛醌10,主要脂肪酸为C16:0、C17:1 ω6c和总特征8(包括C18:1 ω7c和/或C18:1 ω6c)。根据 DNA-DNA 杂交结果、表型、基因型和化学分类数据,分离的菌株 MAH-20T 代表了一个新物种,其名称为 Sphinomonas horti sp。十一月 提出,以 MAH-20T 作为类型菌株(= KACC 19746T = CGMCC1.13658T)。350,026 bp(25 个重叠群),用 3210 个蛋白质编码基因、46 个 tRNA 和 3 个 rRNA 基因注释。分离株的基因组DNA G+C含量为67.3 mol%,主要的醌为泛醌10,主要脂肪酸为C16:0、C17:1 ω6c和总特征8(包括C18:1 ω7c和/或C18:1 ω6c)。根据 DNA-DNA 杂交结果、表型、基因型和化学分类数据,分离的菌株 MAH-20T 代表了一个新物种,其名称为 Sphinomonas horti sp。十一月 提出,以 MAH-20T 作为类型菌株(= KACC 19746T = CGMCC1.13658T)。1 ω7c 和/或 C18:1 ω6c)。根据 DNA-DNA 杂交结果、表型、基因型和化学分类数据,分离的菌株 MAH-20T 代表了一个新物种,其名称为 Sphinomonas horti sp。十一月 提出,以 MAH-20T 作为类型菌株(= KACC 19746T = CGMCC1.13658T)。1 ω7c 和/或 C18:1 ω6c)。根据 DNA-DNA 杂交结果、表型、基因型和化学分类数据,分离的菌株 MAH-20T 代表了一个新物种,其名称为 Sphinomonas horti sp。十一月 提出,以 MAH-20T 作为类型菌株(= KACC 19746T = CGMCC1.13658T)。
更新日期:2020-09-24
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