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Sotolon is a natural virulence mitigating agent in Serratia marcescens
Archives of Microbiology ( IF 2.3 ) Pub Date : 2020-09-24 , DOI: 10.1007/s00203-020-02039-y
Hisham A Abbas 1 , Reham M Goda 2
Affiliation  

Serratia marcescens is an emerging opportunistic bacterium that can cause healthcare-associated infections. The high rate of multidrug resistance and the ability to produce a set of virulence factors, by which it can produce infectious diseases makes it urgent to find an alternative approach to the treatment of such infections. Disarming of virulence by targeting of quorum sensing (QS) as the regulating mechanism of virulence is a promising approach that has no effect on bacterial growth that is considered a key factor in emergence of resistance. This study was designed to investigate the ability of sub-inhibitory concentrations (sub-MICs) of sotolon to attenuate virulence of a clinical isolate of S. marcescens. Sotolon at 25 and 50 μg/ml inhibited 35.2 and 47.5% of biofilm formation, respectively. The inhibition of swimming motility were 41.4 and 69.3%, while that of swarming motility were 77.6 and 86.8% at 25 and 50 µg/ml, respectively. Moreover, sotolon reduced prodigiosin production by 76.6 and 87.6% at concentrations of 25 and 50 µg/ml, respectively. Protease activity was reduced by 25 µg/ml of sotolon by 54.8% and was completely blocked at 50 µg/ml. The relative expression of genes regulating virulence factors decreased by 40% for fimA, 29% for fimC, 59% for flhC, 57% for flhD, 39% for bsmB, 37% for rssB, 49% for rsmA, 54% for pigP, and 62% for shlA gene in the presence of 50 µg/ml sotolon. In conclusion, sotolon is an anti-virulence agent that could be used for the treatment of S.marcescens hospital-acquired infections.

中文翻译:

Sotolon 是粘质沙雷氏菌中的天然毒力减轻剂

粘质沙雷氏菌是一种新兴的机会性细菌,可导致医疗保健相关感染。多重耐药性的高比率和产生一组毒力因子的能力,通过这些因子它可以产生传染病,因此迫切需要找到治疗此类感染的替代方法。通过将群体感应 (QS) 靶向作为毒力调节机制来解除毒力是一种很有前景的方法,它对细菌生长没有影响,而细菌生长被认为是出现耐药性的关键因素。本研究旨在研究 sotolon 的亚抑制浓度 (sub-MIC) 减弱粘质沙门氏菌临床分离株毒力的能力。25 和 50 μg/ml 的 Sotolon 分别抑制了 35.2% 和 47.5% 的生物膜形成。游泳运动抑制为41。4 和 69.3%,而在 25 和 50 µg/ml 时,蜂群运动的分别为 77.6 和 86.8%。此外,sotolon 分别在 25 和 50 µg/ml 的浓度下将灵菌红素的产量降低了 76.6% 和 87.6%。蛋白酶活性被 25 µg/ml sotolon 降低 54.8%,并在 50 µg/ml 时被完全阻断。调节毒力因子的基因的相对表达量,fimA下降40%,fimC下降29%,flhC下降59%,flhD下降57%,bsmB下降39%,rssB下降37%,rsmA下降49%,pigP下降54%,在 50 µg/ml sotolon 存在下,shlA 基因为 62%。总之,sotolon 是一种抗毒力剂,可用于治疗 S.marcescens 医院获得性感染。浓度分别为 25 和 50 µg/ml 时为 6%。蛋白酶活性被 25 µg/ml sotolon 降低 54.8%,并在 50 µg/ml 时被完全阻断。调节毒力因子的基因的相对表达量,fimA下降40%,fimC下降29%,flhC下降59%,flhD下降57%,bsmB下降39%,rssB下降37%,rsmA下降49%,pigP下降54%,在 50 µg/ml sotolon 存在下,shlA 基因为 62%。总之,sotolon 是一种抗毒力剂,可用于治疗 S.marcescens 医院获得性感染。浓度分别为 25 和 50 µg/ml 时为 6%。蛋白酶活性被 25 µg/ml sotolon 降低 54.8%,并在 50 µg/ml 时被完全阻断。调节毒力因子的基因的相对表达量,fimA下降40%,fimC下降29%,flhC下降59%,flhD下降57%,bsmB下降39%,rssB下降37%,rsmA下降49%,pigP下降54%,在 50 µg/ml sotolon 存在下,shlA 基因为 62%。总之,sotolon 是一种抗毒力剂,可用于治疗 S.marcescens 医院获得性感染。在 50 µg/ml sotolon 存在下,pigP 为 54%,shlA 基因为 62%。总之,sotolon 是一种抗毒力剂,可用于治疗 S.marcescens 医院获得性感染。在 50 µg/ml sotolon 存在下,pigP 为 54%,shlA 基因为 62%。总之,sotolon 是一种抗毒力剂,可用于治疗 S.marcescens 医院获得性感染。
更新日期:2020-09-24
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