A bacterial strain, designated CJ1-R5T, was isolated from the flower of the royal azalea plant (Rhododendron schlippenbachii) collected in Jeju Island, Republic of Korea. The strain was a Gram-negative, strictly aerobic, motile, rod-shaped bacterium, growing at a temperature range of 4-33 °C (optimum 28-30 °C), pH 5.0-9.0 (optimum pH 7.0-8.0), and 0-1% NaCl (optimum 0%). The 16S rRNA sequence analysis of strain CJ1-R5T revealed the highest sequence similarity (97.9%) with Xylophilus ampelinus ATCC 33914T, and sequence similarities of less than 97.2% with other validly named species. Phylogenetic tree analysis based on the 16S rRNA gene sequences showed that strain CJ1-R5T clustered with Xylophilus ampelinus ATCC 33914T and two uncultured bacterial clones. The only quinone observed in strain CJ1-R5T was ubiquinone-8. The polar lipids observed were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and two unidentified lipids. The major fatty acids were C16:0, C17:0 cyclo, and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The genome size of strain CJ1-R5T was 5.85 Mbp. The genomic G + C content was 68.4 mol%. ANI and dDDH values between strain CJ1-R5T and Xylophilus ampelinus ATCC 33914T were 79.0% and 22.5%, respectively. Based on the polyphasic taxonomic data, strain CJ1-R5T is considered to represent a novel species, for which the name Xylophilus rhododendri sp. nov. is proposed. The type strain is CJ1-R5T (= KACC 21265T = CCTCC AB2020030T).

中文翻译：

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Xylophilus rhododendri sp. 十一月，从杜鹃花杜鹃花中分离出来

从大韩民国济州岛收集的杜鹃花植物（Rhododendron schlippenbachii）的花中分离出一种细菌菌株，命名为 CJ1-R5T。该菌株为革兰氏阴性、严格需氧、能动、杆状细菌，在 4-33°C（最佳 28-30°C）、pH 5.0-9.0（最佳 pH 7.0-8.0）的温度范围内生长，和 0-1% NaCl（最佳 0%）。菌株CJ1-R5T的16S rRNA序列分析显示，与松材线虫ATCC 33914T的序列相似度最高（97.9%），与其他有效命名物种的序列相似度小于97.2%。基于 16S rRNA 基因序列的系统发育树分析表明，菌株 CJ1-R5T 与 Xylophilus ampelinus ATCC 33914T 和两个未培养的细菌克隆聚类。在菌株 CJ1-R5T 中观察到的唯一醌是泛醌-8。观察到的极性脂质是二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的氨基磷脂和两种未鉴定的脂质。主要脂肪酸是 C16:0、C17:0 环和总特征 8（C18:1 ω7c 和/或 C18:1 ω6c）。菌株 CJ1-R5T 的基因组大小为 5.85 Mbp。基因组 G + C 含量为 68.4 mol%。菌株 CJ1-R5T 和松材线虫 ATCC 33914T 之间的 ANI 和 dDDH 值分别为 79.0% 和 22.5%。根据多相分类数据，菌株 CJ1-R5T 被认为代表了一个新物种，其名称为 Xylophilus rhododendri sp。十一月 被提议。类型菌株是 CJ1-R5T (= KACC 21265T = CCTCC AB2020030T)。主要脂肪酸是 C16:0、C17:0 环和总特征 8（C18:1 ω7c 和/或 C18:1 ω6c）。菌株 CJ1-R5T 的基因组大小为 5.85 Mbp。基因组 G + C 含量为 68.4 mol%。菌株 CJ1-R5T 和松材线虫 ATCC 33914T 之间的 ANI 和 dDDH 值分别为 79.0% 和 22.5%。根据多相分类数据，菌株 CJ1-R5T 被认为代表了一个新物种，其名称为 Xylophilus rhododendri sp。十一月 被提议。类型菌株是 CJ1-R5T (= KACC 21265T = CCTCC AB2020030T)。主要脂肪酸是 C16:0、C17:0 环和总特征 8（C18:1 ω7c 和/或 C18:1 ω6c）。菌株 CJ1-R5T 的基因组大小为 5.85 Mbp。基因组 G + C 含量为 68.4 mol%。菌株 CJ1-R5T 和松材线虫 ATCC 33914T 之间的 ANI 和 dDDH 值分别为 79.0% 和 22.5%。根据多相分类数据，菌株 CJ1-R5T 被认为代表了一个新物种，其名称为 Xylophilus rhododendri sp。十一月 被提议。类型菌株是 CJ1-R5T (= KACC 21265T = CCTCC AB2020030T)。菌株 CJ1-R5T 被认为代表了一种新物种，其名称为 Xylophilus rhododendri sp。十一月 被提议。类型菌株是 CJ1-R5T (= KACC 21265T = CCTCC AB2020030T)。菌株 CJ1-R5T 被认为代表了一种新物种，其名称为 Xylophilus rhododendri sp。十一月 被提议。类型菌株是 CJ1-R5T (= KACC 21265T = CCTCC AB2020030T)。