Grass carps were exposed to the established lethal concentration (LC) values of copper (Cu), chromium (Cr), and lead (Pb) each for the exposed period of 24, 48, 72, and 96 h respectively. Concentrations of these metals were determined in the brain, liver, muscles, gills, kidneys, and intestinal tissues of exposed and control fish through the atomic absorption spectrophotometer after the wet digestion process. The metals accumulation inside these tissues confirmed the absorption of metals from media into the tissues of the model organism. The accumulated concentration in fish tissues was confirmed to be concentration-dependent with significant (p < 0.05) elevated mean values seen for the lead followed by chromium and copper as compared with the mean concentration values of their respective control group. Levels of metals were found above the permissible standards suggested by the regulatory authorities in the fish’s body. Histological sections of the same targeted organs exposed to the three exposure concentration groups were studied and compared with the sections of the healthy group. The histopathological lesions were scored to rank the deleterious effects of metals. The histopathological changes were recorded in concentration and progressive time-related series where gills had the greatest number of scored lesions followed by the kidneys and intestines, muscles, brain, and finally the liver as the least affected organ. Moreover, the organs were not affected uniformly by the metals; in fact, every studied organ has given mild to severe responses towards the toxic metals where lead had proven to cause more severe lesions as compared with copper and chromium. The histological lesions recorded mostly were thus concentration-dependent as revealed in the bioaccumulation of these metals with the effects ranked as lead > chromium > copper with a few exceptions. The findings can be used as a benchmark for the evaluation of the fate and effects of the toxic metals in the expanded aquaculture production of grass carp nationwide. Further investigations with respect to other potentially toxic metals like arsenic, mercury, and cadmium could address the problem towards additional studies.

草鱼分别暴露于既定的铜 (Cu)、铬 (Cr) 和铅 (Pb) 致死浓度 (LC) 值，暴露时间分别为 24、48、72 和 96 小时。在湿消化过程后，通过原子吸收分光光度计测定暴露和对照鱼的脑、肝脏、肌肉、鳃、肾脏和肠道组织中这些金属的浓度。这些组织内的金属积累证实了金属从培养基中吸收到模型生物的组织中。证实鱼组织中的累积浓度呈浓度依赖性，显着（p < 0.05) 与各自对照组的平均浓度值相比，铅其次是铬和铜的平均值升高。发现鱼体内的金属含量高于监管机构建议的允许标准。对暴露于三个暴露浓度组的同一靶器官的组织切片进行了研究，并与健康组的切片进行了比较。对组织病理学损伤进行评分以对金属的有害作用进行排序。组织病理学变化记录在浓度和进行性时间相关序列中，其中鳃具有最多的得分病变，其次是肾脏和肠道、肌肉、大脑，最后是肝脏是受影响最小的器官。此外，器官不受金属的影响。事实上，每个研究过的器官都对有毒金属做出了轻微到严重的反应，与铜和铬相比，铅已被证明会引起更严重的损伤。因此，记录的组织学损伤大多是浓度依赖性的，如这些金属的生物积累所揭示的，其影响排名为铅 > 铬 > 铜，只有少数例外。研究结果可作为评估全国草鱼扩大养殖生产中有毒金属的归宿和影响的基准。对砷、汞和镉等其他潜在有毒金属的进一步调查可以解决这个问题，以进行更多研究。每个被研究的器官都对有毒金属做出轻微到严重的反应，与铜和铬相比，铅已被证明会引起更严重的损伤。因此，记录的组织学损伤大多是浓度依赖性的，如这些金属的生物积累所揭示的，其影响排名为铅 > 铬 > 铜，只有少数例外。研究结果可作为评估全国草鱼扩大养殖生产中有毒金属的归宿和影响的基准。对砷、汞和镉等其他潜在有毒金属的进一步调查可以解决这个问题，以进行更多研究。每个被研究的器官都对有毒金属做出轻微到严重的反应，与铜和铬相比，铅已被证明会引起更严重的损伤。因此，记录的组织学损伤大多是浓度依赖性的，如这些金属的生物积累所揭示的，其影响排名为铅 > 铬 > 铜，只有少数例外。研究结果可作为评估全国草鱼扩大养殖生产中有毒金属的归宿和影响的基准。对砷、汞和镉等其他潜在有毒金属的进一步调查可以解决这个问题，以进行更多研究。因此，记录的组织学损伤大多是浓度依赖性的，如这些金属的生物积累所揭示的，其影响排名为铅 > 铬 > 铜，只有少数例外。研究结果可作为评估全国草鱼扩大养殖生产中有毒金属的归宿和影响的基准。对砷、汞和镉等其他潜在有毒金属的进一步调查可以解决这个问题，以进行更多研究。因此，记录的组织学损伤大多是浓度依赖性的，如这些金属的生物积累所揭示的，其影响排名为铅 > 铬 > 铜，只有少数例外。研究结果可作为评估全国草鱼扩大养殖生产中有毒金属的归宿和影响的基准。对砷、汞和镉等其他潜在有毒金属的进一步调查可以解决这个问题，以进行更多研究。