Human dermal fibroblast proliferation plays an important role in skin wound healing, and electrical stimulation (ES) promotes skin wound healing. Although the use of ES for skin wound healing has been investigated, the mechanism underlying the effects of ES on cells is still unclear. This study examined the effects of pulsed electrical stimulation (PES) on human dermal fibroblasts. Normal adult human dermal fibroblasts were exposed to a frequency of 4800 Hz, voltage of 1-5 V, and PES exposure time of 15, 30, and 60 min. Dermal fibroblast proliferation and growth factor gene expression were investigated for 6-48 h post PES. Dermal fibroblast proliferation significantly increased from 24 to 48 h post PES at a voltage of 5 V and PES exposure time of 60 min. Under the same conditions, post PES, platelet-derived growth factor subunit A (PDGFA), fibroblast growth factor 2 (FGF2), and transforming growth factor beta 1 (TGF-β1) expression significantly increased from 6 to 24 h, 12 to 48 h, and 24 to 48 h, respectively. Imatinib, a specific inhibitor of platelet-derived growth factor receptor, significantly inhibited the proliferation of dermal fibroblasts promoted by PES, suggesting that PDGFA expression, an early response of PES, was involved in promoting the cell proliferation. Therefore, PES at 4800 Hz may initially promote PDGFA expression and subsequently stimulate the expression of two other growth factors, resulting in dermal fibroblast proliferation after 24 h or later. In conclusion, PES may activate the cell growth phase of wound healing.

中文翻译：

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脉冲电刺激对人真皮成纤维细胞生长因子基因表达和增殖的影响。

人真皮成纤维细胞增殖在皮肤伤口愈合中发挥重要作用，电刺激（ES）可促进皮肤伤口愈合。尽管已经研究了 ES 用于皮肤伤口愈合的用途，但 ES 对细胞影响的机制仍不清楚。这项研究探讨了脉冲电刺激 (PES) 对人类真皮成纤维细胞的影响。正常成人真皮成纤维细胞暴露于 4800 Hz 的频率、1-5 V 的电压和 15、30 和 60 分钟的 PES 暴露时间。PES 后 6-48 小时研究真皮成纤维细胞增殖和生长因子基因表达。在电压为 5 V、PES 暴露时间为 60 分钟的情况下，PES 后 24 至 48 小时，真皮成纤维细胞增殖显着增加。相同条件下，PES后，血小板源性生长因子亚基A（PDGFA）、成纤维细胞生长因子2（FGF2）和转化生长因子β1（TGF-β1）表达从6小时至24小时、12小时至48小时显着增加小时和24至48小时，分别。血小板源性生长因子受体特异性抑制剂伊马替尼显着抑制PES促进的真皮成纤维细胞的增殖，表明PDGFA表达是PES的早期反应，参与促进细胞增殖。因此，4800 Hz 的 PES 可能首先促进 PDGFA 表达，随后刺激其他两种生长因子的表达，导致 24 小时或更晚后真皮成纤维细胞增殖。总之，PES 可能激活伤口愈合的细胞生长期。