Syncytin-1 (gene ERVW-1) has been proposed as a marker of pre-eclampsia and malfunctions in placental development. Placenta is heterogeneous tissue, hence the method of biopsy can significantly affect the outcome of analyses. A total of 44 placentae were analyzed by taking 3-30 samples from each. Relative levels of ERVW-1 expression in the placental biopsies were characterized by RT-qPCR. Evaluation of ten biopsies from one placenta individually (not pooling them) is recommended due to the high variability of expression. No significant correlation was found between biopsy localization and level of ERVW-1 expression; therefore, random sampling is recommended. A long cut from the umbilical cord to the edge of the placenta is a convenient approach to placental sampling.

中文翻译：

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用于合胞素 1 分析的适当胎盘取样。

Syncytin-1（基因ERVW-1）已被提议作为先兆子痫和胎盘发育障碍的标志物。胎盘是异质组织，因此活检方法会显着影响分析结果。通过从每个胎盘中取出 3-30 个样品分析了总共 44 个胎盘。胎盘活检中ERVW-1表达的相对水平通过 RT-qPCR 进行表征。由于表达的高度可变性，建议单独评估来自一个胎盘的 10 个活组织检查（而不是合并它们）。活检定位与ERVW-1水平之间未发现显着相关性表达; 因此，建议随机抽样。从脐带到胎盘边缘的长切口是一种方便的胎盘取样方法。