In this work the potential of comparative transcriptomics was explored of Saccharomyces (S.) cerevisiae and S. pastorianus for their discrimination. This way an alternative should be demonstrated to comparative genomics, which can be difficult as a result of their aneuoploid genomes composed of mosaics of the parental genomes. Strains were selected according to their application in beer brewing, i.e. top and bottom fermenting yeasts. Comparative transcriptomics was performed for four strains each of commercially available S. cerevisiae (top fermenting) and Saccharomyces pastorianus (bottom fermenting) brewing yeasts grown at two different temperatures to mid-exponential growth phase. A non-reference based approach was chosen in the form of alignment against a de novo assembled brewery-associated pan transcriptome to exclude bias introduced by manual selection of reference genomes. The result is an analysis workflow for self-contained comparative transcriptomics of Saccharomyces yeasts including, but not limited to, the analysis of core and accessory gene expression, functional analysis and metabolic classification. The functionality of this workflow is demonstrated along the principal differentiation of accessory transcriptomes of S. cerevisiae versus S. pastorianus strains. Hence, this work provides a concept enabling studies under different brewing conditions.

中文翻译：

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探索从头比较转录组学对酿酒酵母酿造酵母进行分类的潜力。

在这项工作中，研究了酿酒酵母和巴斯德酵母的比较转录组学的潜力。这样，应该证明比较基因组学的另一种选择，由于其非亲倍体基因组由亲本基因组的镶嵌组成，可能比较困难。根据其在啤酒酿造中的应用选择菌株，即顶部和底部发酵酵母。对四种菌株进行了比较转录组学，这些菌株分别在两个不同的温度下生长至指数中期生长阶段，分别购得啤酒酵母（顶部发酵）和巴斯德酿酒酵母（底部发酵）酿造酵母。选择一种基于非参考的方法，以与从头组装啤酒厂相关的泛转录组比对的形式选择，以排除因手动选择参考基因组而引起的偏倚。结果是用于酿酒酵母的自包含比较转录组学的分析工作流程，包括但不限于核心和辅助基因表达分析，功能分析和代谢分类。沿酿酒酵母菌株与巴斯德酵母菌株的辅助转录组的主要区别证明了该工作流程的功能。因此，这项工作提供了一个概念，使得可以在不同的酿造条件下进行研究。但不限于核心和辅助基因表达分析，功能分析和代谢分类。沿酿酒酵母菌株与巴斯德酵母菌株的辅助转录组的主要区别证明了该工作流程的功能。因此，这项工作提供了一个概念，使得可以在不同的酿造条件下进行研究。但不限于核心和辅助基因表达分析，功能分析和代谢分类。沿酿酒酵母菌株与巴斯德酵母菌株的辅助转录组的主要区别证明了该工作流程的功能。因此，这项工作提供了一个概念，使得可以在不同的酿造条件下进行研究。