Serum levels of interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 6 (IL-6), interleukin 10 (IL-10), interleukin 17 (IL-17), interferon gamma (IFN-γ), tumor necrosis factor α (TNF-α), and interleukin 1β (IL-1β), cytokines involved in the immune response, were investigated in 75 Leishmania-positive blood donors living in endemic areas. Based on their status in 2011 and 2015, the subjects were clustered into three groups: positive for at least one diagnostic method in both years, but lacking clinical progression to disease (G1); positive on at least one method in 2011 but negative in 2015 (G2); negative on all methods in both years (G3). Donors were interviewed for sociodemographic data collection and underwent clinical evaluation and laboratory tests. Serum cytokines were quantified using a CBA Flex set (BD Biosciences). Significant differences were found for all the cytokines evaluated, with lower concentrations in consistently Leishmania-negative individuals. The exception was IFN-γ, with similar levels among all donors. No changes consistent with active disease were observed in the laboratory results for Leishmania-positive donors who underwent clinical evaluation, none of whom progressed to disease. This suggests that infection control is associated with serum IL-17 levels. Resolution of Leishmania infection in positive donors may be related to high levels of IL-17 and low levels of IL-10, highlighting the role played by IL-17 in asymptomatic Leishmania-infected individuals.

中文翻译：

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利什曼原虫阳性献血者的细胞因子谱。

血清白介素2（IL-2），白介素4（IL-4），白介素6（IL-6），白介素10（IL-10），白介素17（IL-17），干扰素γ（IFN-γ）在生活在流行地区的75名利什曼原虫阳性献血者中，研究了肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1β）等参与免疫反应的细胞因子。根据他们在2011年和2015年的状况，将受试者分为三组：两年中至少一种诊断方法呈阳性，但缺乏疾病进展（G1）；在2011年对至少一种方法表示肯定，但在2015年表示否定（G2）；两年中所有方法的结果均为阴性（G3）。对捐赠者进行了社会人口统计学数据收集采访，并进行了临床评估和实验室测试。使用CBA Flex套件（BD Biosciences）定量血清细胞因子。发现所有评估的细胞因子都有显着差异，在利什曼原虫阴性患者中浓度较低。例外是IFN-γ，所有供体之间的水平相似。在接受临床评估但未进展为疾病的利什曼原虫阳性供体的实验室结果中，未观察到与活动性疾病相符的变化。这表明感染控制与血清IL-17水平有关。阳性供体中利什曼原虫感染的消退可能与高水平的IL-17和低水平的IL-10有关，这突显了IL-17在无症状利什曼原虫感染个体中发挥的作用。在接受临床评估但未进展为疾病的利什曼原虫阳性供体的实验室结果中，未观察到与活动性疾病相符的变化。这表明感染控制与血清IL-17水平有关。阳性供体中利什曼原虫感染的消退可能与高水平的IL-17和低水平的IL-10有关，这突显了IL-17在无症状利什曼原虫感染个体中发挥的作用。在接受临床评估但未进展为疾病的利什曼原虫阳性供体的实验室结果中，未观察到与活动性疾病相符的变化。这表明感染控制与血清IL-17水平有关。阳性供体中利什曼原虫感染的消退可能与高水平的IL-17和低水平的IL-10有关，这突显了IL-17在无症状利什曼原虫感染个体中发挥的作用。