Destruction of myelin, or demyelination, is a characteristic of traumatic spinal cord injury and pathognomonic for primary demyelinating pathologies such as multiple sclerosis (MS). The regenerative process known as remyelination, which can occur following demyelination, fails as MS progresses. Models of focal demyelination by local injection of gliotoxins have provided important biological insights into the demyelination/remyelination process. Here, injection of lysolecithin to induce spinal cord demyelination is investigated using matrix-assisted laser desorption/ionization mass spectrometry imaging. A segmentation analysis revealed changes to the lipid composition during lysolecithin-induced demyelination at the lesion site and subsequent remyelination over time. The results of this study can be utilized to identify potential myelin-repair mechanisms and in the design of therapeutic strategies to enhance myelin repair.

中文翻译：

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MALDI 质谱成像在小鼠脊髓的初级脱髓鞘模型中。

髓鞘破坏或脱髓鞘是外伤性脊髓损伤的特征，是原发性脱髓鞘病变（如多发性硬化症 (MS)）的特征。被称为髓鞘再生的再生过程会在脱髓鞘后发生，但会随着 MS 的进展而失败。通过局部注射胶质毒素的局灶性脱髓鞘模型为脱髓鞘/髓鞘再生过程提供了重要的生物学见解。在这里，使用基质辅助激光解吸/电离质谱成像研究了注射溶血卵磷脂以诱导脊髓脱髓鞘。分段分析揭示了溶血卵磷脂诱导的病变部位脱髓鞘和随后的髓鞘再生随着时间的推移脂质成分的变化。