Woody biomass represents an important source of carbon on earth, and its global recycling is highly dependent on Agaricomycetes fungi. White-rot Basidiomycetes are a very important group in this regard, as they possess a large and diverse enzymatic repertoire for biomass decomposition. Among these enzymes, the recently discovered lytic polysaccharide monooxygenases (LPMOs) have revolutionized biomass processing with their novel oxidative mechanism of action. The strikingly high representation of LPMOs in fungal genomes raises the question of their functional versatility. In this work, we studied an AA9 LPMO from the white-rot basidiomycete Pycnoporus sanguineus, PsAA9A. Successfully produced as a recombinant secreted protein in Pichia pastoris, PsAA9A was found to be a C1-specific LPMO active on cellulosic substrates, generating native and oxidized cello-oligosaccharides in the presence of an external electron donor. PsAA9A boosted cellulolytic activity of glysoside hydrolases from families GH1, GH5, and GH6.This study serves as a starting point towards understanding the functional versatility and biotechnological potential of this enzymatic family, highly represented in wood decay fungi, in Pycnoporus genus. KEY POINTS: • PsAA9A is the first AA9 from P. sanguineus to be characterized. • PsAA9A has activity on cellulose, producing C1-oxidized cello-oligosaccharides. • Boosting activity with GH1, GH5, and GH6 was proven.

中文翻译：

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PsAA9A，一种来自C1特异AA9的溶多糖单加氧酶，来自白腐烂担子菌碧萝y（Pycnoporus sanguineus）。

木质生物质是地球上重要的碳源，其全球循环利用高度依赖于姬松茸真菌。在这方面，白腐烂担子菌是一个非常重要的群体，因为它们拥有大量多样的生物质分解酶库。在这些酶中，最近发现的裂解多糖单加氧酶（LPMO）以其新颖的氧化作用机制彻底改变了生物质的加工过程。LPMO在真菌基因组中的显着高表达提出了其功能多样性的问题。在这项工作中，我们研究了来自白腐烂担子菌Pycnoporus sanguineus PsAA9A的AA9 LPMO。PsAA9A已成功生产为巴斯德毕赤酵母中的重组分泌蛋白，是一种在纤维素底物上具有活性的C1特异性LPMO，在外部电子供体的存在下产生天然的和氧化的纤维寡糖。PsAA9A增强了GH1，GH5和GH6家族的糖苷水解酶的纤维素分解活性。本研究为了解该酶家族的功能通用性和生物技术潜力提供了起点，该酶家族在碧萝op属中以木材腐烂真菌为代表。要点：•PsAA9A是血红假单胞菌中第一个被鉴定的AA9。•PsAA9A对纤维素具有活性，可产生C1氧化的纤维寡糖。•已证明使用GH1，GH5和GH6具有增强活性。这项研究为了解该酶家族的功能多功能性和生物技术潜力提供了起点，该酶家族以碧萝decay属的木材腐烂真菌为代表。要点：•PsAA9A是血红假单胞菌中第一个被鉴定的AA9。•PsAA9A对纤维素具有活性，可产生C1氧化的纤维寡糖。•已证明使用GH1，GH5和GH6具有增强活性。这项研究为了解该酶家族的功能多功能性和生物技术潜力提供了起点，该酶家族以碧萝decay属的木材腐烂真菌为代表。要点：•PsAA9A是血红假单胞菌中第一个被鉴定的AA9。•PsAA9A对纤维素具有活性，可产生C1氧化的纤维寡糖。•已证明使用GH1，GH5和GH6具有增强活性。