AIM To evaluate the efficacy of a prototype root canal dressing containing surface pre-reacted glass-ionomer (S-PRG) fillers on repairing induced periapical lesions in a rat model. Calcium hydroxide [Ca(OH)2 ] was applied as a comparison in the healing process. METHODOLOGY The pulp chambers of the maxillary first molars in 64 male Wistar rats aged 16 weeks were opened to induce periapical lesions. After 28 days, the mesial canal of each tooth was prepared, irrigated with 2.5% sodium hypochlorite (NaOCl) only (control group: irrigation) or followed by the respective dressing [Ca(OH)2 group, irrigation + Ca(OH)2 ; S-PRG group, irrigation + S-PRG], and restored with composite resin for 3 or 7 days (10/group). Four rats with healthy molars were used as blank controls. Descriptive analysis of the periapical radiographs, haematoxylin and eosin staining, and immunohistochemical observation were performed 3 and 7 days after treatment. The periapical gray value, CD68 macrophages, and osteoclasts (cathepsin-K) were quantified and statistically analyzed with Tukey's honest significant difference test. A significant difference was achieved when p values were < 0.05. RESULTS S-PRG and Ca(OH)2 dressing were associated with increased periapical gray value and inhibited osteoclast activity at 3 and 7 days; a significant difference in radiographic results and the number of osteoclasts was obtained at 3 and 7 days compared with the control group (p < 0.05). Reparative tissue was observed histologically in the space of the periapical resorbed necrotic area after S-PRG and Ca(OH)2 treatment for 3 and 7 days. The number of macrophages was significantly decreased at 3 and 7 days in the S-PRG and Ca(OH)2 specimens when compared with the controls (p < 0.05). CONCLUSIONS In a rat experimental model, the S-PRG root canal dressing was comparable to Ca(OH)2 in promoting experimentally induced periapical lesion healing. S-PRG paste has the potential to be used as an alternative intracanal dressing in teeth with apical periodontitis.

中文翻译：

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S-PRG根管敷料治疗大鼠根尖周炎的潜力。

目的评估包含表面预反应的玻璃离聚物（S-PRG）填料的原型根管敷料在大鼠模型中修复诱导的根尖周病变的功效。氢氧化钙[Ca（OH）2]用于比较愈合过程。方法学打开64只16周龄Wistar雄性大鼠的上颌第一磨牙的牙髓腔，以诱发根尖周病变。28天后，准备好每颗牙齿的中耳道，仅用2.5％次氯酸钠（NaOCl）进行灌溉（对照组：冲洗），或随后分别进行敷料治疗[Ca（OH）2组，冲洗+ Ca（OH）2 ; S-PRG组，灌溉+ S-PRG]，并用复合树脂恢复3或7天（每组10个）。将四只具有健康磨牙的大鼠用作空白对照。根尖周片，苏木精和曙红染色的描述性分析，治疗后3、7天进行免疫组化观察。使用Tukey的诚实显着差异检验对根尖周灰度值，CD68巨噬细胞和破骨细胞（cathepsin-K）进行定量和统计分析。当p值<0.05时，差异显着。结果S-PRG和Ca（OH）2敷料与根尖周灰值增加和在第3天和第7天抑制破骨细胞活性有关；与对照组相比，第3天和第7天的放射学结果和破骨细胞的数量有显着差异（p <0.05）。S-PRG和Ca（OH）2处理3天和7天后，在根尖周吸收的坏死区域的空间组织学观察到修复组织。与对照组相比，S-PRG和Ca（OH）2标本在第3天和第7天巨噬细胞数量显着减少（p <0.05）。结论在大鼠实验模型中，S-PRG根管敷料在促进实验诱导的根尖周病变愈合方面与Ca（OH）2相当。S-PRG糊剂有可能在根尖性牙周炎的牙齿中用作替代的管内敷料。