Corn, sorghum and wheat grains are used as livestock feed in the world. Identification of black aspergilli associated with these grains is necessary to make sure of the safety of the grains because its occurrence is an indicator of mycotoxin production. Forty-five isolates were isolated from the samples collected from Upper Egypt’s markets and identified morphologically based on colony color, conidia, stipe and vesicle size and molecularly by using β-tubulin and calmodulin genes. Isolates were divided into 30 strains of Aspergillus welwitschiae and 15 strains of A. niger . We have found new criteria in the morphological identification of A. welwitschiae as its colony color was black to brown with yellow edge, but in A. niger was black with white edge, also A. welwitschiae sometimes produced finely-to-distinctly roughened brownish conidia on malt extract agar (MEA) media. Thirteen isolates of A. welwitschiae and six of A. niger were recognized as potential producers for ochratoxin A.

中文翻译：

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玉米、高粱和小麦籽粒黑曲霉聚集体的新形态学标准和分子表征

玉米、高粱和小麦谷物在世界上被用作牲畜饲料。鉴定与这些谷物相关的黑曲霉对于确保谷物的安全性是必要的，因为它的出现是霉菌毒素产生的一个指标。从上埃及市场收集的样品中分离出 45 个分离株，并根据菌落颜色、分生孢子、菌柄和囊泡大小进行形态学鉴定，并使用 β-微管蛋白和钙调蛋白基因进行分子鉴定。分离物被分为30 株曲霉 welwitschiae 和 15 株黑曲霉。我们在 A. welwitschiae 的形态学鉴定中找到了新的标准，因为其菌落颜色为黑色至棕色，边缘为黄色，但在 A. niger 中为黑色，边缘为白色，A. welwitschiae 有时在麦芽提取物琼脂 (MEA) 培养基上产生精细到明显粗糙的褐色分生孢子。13 株 A. welwitschiae 和 6 株 A. niger 被认为是赭曲霉毒素 A 的潜在生产者。