Age-related macular degeneration is an incurable chronic neurodegenerative disease, causing progressive loss of the central vision and even blindness. Up-to-date therapeutic approaches can only slow down he progression of the disease. Objective. Feasibility study for a multilayered, silk fibroin-based, 3D biohybrid retina. Approach . Fabrication of silk fibroin-based biofilms; culture of different types of cells: retinal pigment epithelium, retinal neurons, Müller and mesenchymal stem cells ; creation of a layered structure glued with silk fibroin hydrogel. Main results . In vitro evidence for the feasibility of layered 3D biohybrid retinas; primary culture neurons grow and develop neurites on silk fibroin biofilms, either alone or in presence of other cells cultivated on the same biomaterial; cell organization and cellular phenotypes are maintained in vitro for the seven days of the experiment. Significance. 3D biohybrid retina can be bui...

中文翻译：

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开发用于年龄相关性黄斑变性 (AMD) 的基于丝素蛋白的 3D 生物杂交视网膜的第一步。

年龄相关性黄斑变性是一种无法治愈的慢性神经退行性疾病，会导致中央视力逐渐丧失甚至失明。最新的治疗方法只能减缓疾病的进展。客观的。多层、基于丝素蛋白的 3D 生物杂交视网膜的可行性研究。方法 。基于丝素蛋白的生物膜的制备；不同类型细胞的培养：视网膜色素上皮细胞、视网膜神经元、Müller 和间充质干细胞；创建与丝素蛋白水凝胶粘合的分层结构。主要结果。分层 3D 生物混合视网膜可行性的体外证据；原代培养神经元在丝素蛋白生物膜上生长和发育轴突，单独或在同一生物材料上培养的其他细胞存在的情况下；在 7 天的实验中，细胞组织和细胞表型在体外保持。意义。3D 生物杂交视网膜可以构建...