Three-dimensional cell culture technology is a novel cell culture technology, which can simulate the growth state of cells in vivo by scaffolds or special devices. Cells can form tissues or organs in vitro. It combines some advantages of traditional cell experiments and animal model experiments. Because of its advantages, it is widely used in clinical medical research, including research on stem cell differentiation, research on cell behavior, migration and invasion, study on microenvironment, study on drug sensitivity and radio-sensitivity of tumor cells, etc. In this paper, the evolution and classification of three-dimensional cell culture are reviewed, also the advantages and shortages are compared. The application of three-dimensional cell culture in clinical medicine are summarized to provide an insight into translational medicine.

中文翻译：

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三维细胞培养在临床医学中的应用

三维细胞培养技术是一种新型的细胞培养技术，可以通过支架或特殊装置模拟细胞在体内的生长状态。细胞可以在体外形成组织或器官。它结合了传统细胞实验和动物模型实验的一些优点。由于其优势，它被广泛应用于临床医学研究，包括干细胞分化研究、细胞行为、迁移和侵袭研究、微环境研究、肿瘤细胞药物敏感性和放射敏感性研究等。论文综述了三维细胞培养的演变和分类，比较了其优缺点。总结了三维细胞培养在临床医学中的应用，以提供对转化医学的深入了解。