Changes in mechanical properties of cells are closely related to a variety of diseases. As an advanced technology on the micro/nano scale, atomic force microscopy is the most suitable tool for information acquisition of living cells in human body fluids. AFMs are able to measure and characterize the mechanical properties of cells which can be used as effective markers to distinguish between different cell types and cells in different states (benign or cancerous). Therefore, they can be employed to obtain additional information to that obtained via the traditional biochemistry methods for better identifying and diagnosing cancer cells for humans, proposing better treatment methods and prognosis, and unravelling the pathogenesis of the disease. In this report, we review the use of AFMs in cancerous tissues, organs, and cancer cells cultured in vitro to obtain cellular mechanical properties, demonstrate and summarize the results of AFMs in cancer biology, and look forward to possible future applications and the direction of development.

中文翻译：

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原子力显微镜在单个癌细胞诊断中的应用

细胞力学性能的变化与多种疾病密切相关。原子力显微镜作为微纳尺度的先进技术，是人体体液中活细胞信息获取最合适的工具。AFM 能够测量和表征细胞的机械特性，可用作区分不同细胞类型和不同状态（良性或癌性）的细胞的有效标记。因此，它们可以用来获得传统生物化学方法之外的额外信息，以便更好地识别和诊断人类癌细胞，提出更好的治疗方法和预后，并揭示疾病的发病机制。在本报告中，我们回顾了AFM在癌组织、器官和体外培养的癌细胞中的应用，以获得细胞力学特性，展示和总结了AFM在癌症生物学中的结果，并展望了未来可能的应用和方向。发展。