Background: Boswellic acids are the main constituents of Boswellia serrata gum. These comprise of four pentacyclic triterpenes, 11-keto-β-boswellic acid (KBA) being one of them.

Objectives: Comparing the extraction efficiency of KBA through microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) followed by optimizing the extraction process using response surface methodology (RSM) and validation of HPTLC.

Methods: UAE and MAE of KBA were carried out employing methanol as an extracting solvent. To figure out the better mode of extraction, single factorial experiments were conducted for further optimization. Design expert software was used for optimization purposes where solvent to drug ratio, extraction temperature and extraction time were taken as input variables. Quantification of KBA in each extract was done through High-Performance Thin Layer Chromatography (HPTLC), and the method was validated as per International Council for Harmonization (ICH) guidelines.

Results: UAE stood out to be a better mode of extraction for KBA. Solvent to drug ratio of 20.42 mL/gram, extraction temperature of 44.01°C and extraction time of 11.54 minutes were established as optimum conditions, which yielded 8.44%w/w of KBA. Regarding HPTLC, the Rf value of KBA was measured, and the correlation coefficient was calculated from the standard curve. Accuracy, precision and recovery were found within limits.

Conclusion: From this study, it was concluded that a non-thermal method is a better choice of extraction for KBA. All the input variables significantly affected KBA content, which was confirmed by model fitting. Moreover, the HPTLC method was developed for the quantification of KBA, which was found to be accurate, reliable and highly sensitive.

背景：乳香酸是乳香锯缘树胶的主要成分。这些包括四种五环三萜，11-酮-β-乳香酸（KBA）是其中之一。

目的：通过微波辅助萃取 (MAE) 和超声辅助萃取 (UAE) 比较 KBA 的萃取效率，然后使用响应面法 (RSM) 优化萃取工艺并验证 HPTLC。

方法：采用甲醇为萃取溶剂，进行了高宝的UAE和MAE。为了找出更好的提取模式，进行了单因子实验以进一步优化。设计专家软件用于优化目的，其中溶剂与药物的比例、提取温度和提取时间作为输入变量。通过高性能薄层色谱 (HPTLC) 对每种提取物中的 KBA 进行定量，该方法已根据国际协调委员会 (ICH) 指南进行了验证。

结果：UAE 脱颖而出，成为 KBA 的一种更好的提取模式。以20.42 mL/g的溶剂与药物比、44.01°C的提取温度和11.54分钟的提取时间为最佳条件，得到8.44%w/w的KBA。关于HPTLC，测量KBA的Rf值，并从标准曲线计算相关系数。准确度、精密度和回收率均在限度内。

结论：从这项研究中得出结论，非热法是提取 KBA 的更好选择。所有输入变量均显着影响 KBA 内容，模型拟合证实了这一点。此外，还开发了用于定量 KBA 的 HPTLC 方法，发现该方法准确、可靠且灵敏度高。