Background: Various effects of Astaxanthin were shown in the studies, including its antioxidant, anti-inflammatory, anti-tumor and immunoregulatory effects.

Objective: The aim of this study was to evaluate the beneficial effects of Astaxanthin on renovascular occlusion induced renal injury and to investigate the possible mechanisms.

Methods: The rats were randomly assigned into three groups as follows: Group 1: control group (n=12), Group 2: renal ischemia-reperfusion injury group (n=12), Group 3: renal ischemia-reperfusion + asthaxantine treated group (n=12). The control group and the renal ischemia-reperfusion group were given 2cc/kg/g olive oil for 7 days before establishing ischemia to renal tissue. Astaxanthin dissolved in olive oil was given orally to the renal ischemia+astaxanthin group for 7 days before inducing renal ischemia. Caspase-(3, 8, 9), GSH, SOD, Total Thiol, TNF-α, IL-6, 8-OHdG were evaluated in each group.

Results: Renal IRI was verified by analysing the pathological changes of renal tissues and the renal functions after renal reperfusion. Much less renal tubular damage was determined in the IRI+ASX group in comparison to the IRI group. Caspase-8, -9 and -3 immunoreactivity was observed to be minimal in the control group. Apoptosis was observed to be significantly reduced in the IRI + ASX group with respect to the IRI group and close to the level of the control group (p <0.05). Caspase-3 levels of tissue samples were significantly increased in the IRI group compared to the other groups, but significantly lower in the IRI+ASX group with respect to the IRI group (p<0.05). The TOS and OSI levels, indicating increased oxidative stress, were significantly lower in the IRI+ASX group with respect to the IRI group (p <0.001), but still higher than the control group (p <0.001). In addition to GSH, SOD and Total Thiol levels, TAS levels were also significantly higher in the IRI + ASX group in comparison to the IRI group (p <0.05). TNF-α, IL-6, lipid hydroperoxide, AOPP and 8-OHdG levels were lower in the IRI+ASX group than the IRI group (p <0.001). MPO, IL-6, TNF-α levels, representing the parameters indicating neutrophil infiltration and inflammation of the renal tissues, significantly increased in the IRI group with respect to the other groups (p <0.005).

Conclusion: When all the data obtained in our study were evaluated, ASX was determined to prevent renal damage due to renovascular occlusion to a great extent, through complex mechanisms involving antioxidant, anti-inflammatory and antiapoptotic effects. Biochemical, histological and oxidative stress parameters were improved due to ASX.

背景：研究显示了虾青素的多种作用，包括其抗氧化、抗炎、抗肿瘤和免疫调节作用。

目的：本研究的目的是评估虾青素对肾血管闭塞性肾损伤的有益作用，并探讨可能的机制。

方法：将大鼠随机分为如下三组：第1组：对照组（n=12），第2组：肾缺血再灌注损伤组（n=12），第3组：肾缺血再灌注+虾青素治疗组（n = 12）。对照组和肾缺血再灌注组在建立肾组织缺血前给予2cc/kg/g橄榄油7天。在诱导肾缺血前，将溶解在橄榄油中的虾青素口服给予肾缺血+虾青素组7天。在每组中评估 Caspase-(3, 8, 9)、GSH、SOD、总硫醇、TNF-α、IL-6、8-OHdG。

结果：通过分析肾组织病理变化及肾再灌注后肾功能，验证肾IRI。与 IRI 组相比，IRI+ASX 组的肾小管损伤要小得多。在对照组中观察到 Caspase-8、-9 和 -3 免疫反应性最低。与 IRI 组相比，IRI + ASX 组的细胞凋亡明显减少，接近对照组的水平（p <0.05）。与其他组相比，IRI 组的组织样本的 Caspase-3 水平显着增加，但 IRI+ASX 组的组织样本水平显着低于 IRI 组（p<0.05）。与 IRI 组相比，IRI+ASX 组的 TOS 和 OSI 水平（表明氧化应激增加）显着降低（p <0.001），但仍高于对照组（p <0.001）。除了 GSH、SOD 和总硫醇水平外，IRI + ASX 组的 TAS 水平也显着高于 IRI 组（p <0.05）。IRI+ASX 组的 TNF-α、IL-6、脂质过氧化氢、AOPP 和 8-OHdG 水平低于 IRI 组（p <0.001）。MPO、IL-6、TNF-α 水平（代表指示中性粒细胞浸润和肾组织炎症的参数）在 IRI 组中相对于其他组显着增加（p <0.005）。0.001)。MPO、IL-6、TNF-α 水平（代表指示中性粒细胞浸润和肾组织炎症的参数）在 IRI 组中相对于其他组显着增加（p <0.005）。0.001)。MPO、IL-6、TNF-α 水平（代表指示中性粒细胞浸润和肾组织炎症的参数）在 IRI 组中相对于其他组显着增加（p <0.005）。

结论：当对我们研究中获得的所有数据进行评估时，ASX 被确定通过涉及抗氧化、抗炎和抗凋亡作用的复杂机制，在很大程度上预防了肾血管闭塞引起的肾损伤。由于 ASX，生化、组织学和氧化应激参数得到改善。