Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals. In humans, in contrast to the cutaneous form called erysipeloid, which is an occupational disease and common in individuals who handle raw meat and fish, invasive systemic infections are unusual. E. rhusiopathiae expresses an immunogenic surface protein, Spa (surface protective antigen), which is involved in virulence. Among the antigenically different Spa proteins (SpaA, B and C), which are mostly associated with serovars, SpaA is by far the most prevalent in E. rhusiopathiae isolates from diseased animals. However, the Spa type has not been examined for human isolates, and it is unknown whether SpaB- or SpaC-possessing isolates can cause disease in humans. A Gram-positive, rod-shaped bacterium isolated from a case of human pyogenic spondylitis was analysed. The bacterium was identified as E. rhusiopathiae by a routine biochemical test and MS, and ultimately confirmed by an E. rhusiopathiae -specific PCR assay. Spa typing by sequencing revealed the SpaB type, and the serovar of the strain was identified as untypeable by a conventional agar gel precipitation test, but determined to be serovar 6 by a serotyping PCR assay. Sequence analysis of the serovar-defining chromosomal region revealed that the isolate displayed the same gene organization as the serovar 6 reference strain, but the region was disrupted by an insertion sequence element, suggesting that the isolate originated from a serovar 6 strain. These results highlight that unusual, spaB-possessing E. rhusiopathiae strains can potentially pose serious risks to humans.

中文翻译：

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一种侵染性感染，在人体内具有不寻常的spaB侵染性红斑丹毒丝菌。

红斑丹毒丝菌 是一种人畜共患病原体，可在多种动物中引起丹毒。在人类中，与称为类红藻的皮肤病相反，后者是一种职业病，在处理生肉和鱼的个体中很常见，而侵入性系统感染则很罕见。大肠埃希氏菌表达一种涉及致病性的免疫原性表面蛋白Spa（表面保护性抗原）。在抗原性不同的Spa蛋白（SpaA，B和C）中，大多数与血清型有关，SpaA是目前在风湿性大肠杆菌中最普遍的 从患病动物中分离出来。但是，尚未对人类分离株的Spa类型进行过检查，尚不清楚具有SpaB或SpaC的分离株是否会导致人类疾病。分析了从人化脓性脊柱炎病例中分离出的革兰氏阳性棒状细菌。该细菌经常规生化检验和质谱鉴定为风湿性大肠埃希菌，最终被风湿性大肠埃希菌确诊 特异性PCR分析。通过测序的Spa分型显示了SpaB类型，并且该菌株的血清型通过常规琼脂凝胶沉淀测试被鉴定为无法分型，但通过血清分型PCR分析确定为血清型6。定义血清型的染色体区域的序列分析显示，该分离株显示出与血清型6参考菌株相同的基因组织，但是该区域被插入序列元件破坏，表明该分离株起源于血清型6菌株。这些结果突出显示异常，SPAB -possessing E.丝菌菌株有可能造成对人体的严重风险。