Methanogenic microbial communities were enriched from rice paddy soil and anaerobic digester sludge using peptidoglycan purified from gram-negative Escherichia coli or gram-positive Micrococcus luteus as the sole substrate. Methane production data suggested the anaerobic degradation of peptidoglycan and also that peptidoglycan from E. coli had lower degradability. The community structures of enrichment cultures fed peptidoglycan from E. coli or M. luteus were similar, but distinctly different. A number of phylogenetically novel and uncultured bacteria, particularly in the phyla Bacteroidetes, WWE1, Armatimonadetes, and Verrucomicrobia, dominated the enrichment cultures, suggesting their involvement in anaerobic peptidoglycan degradation.

中文翻译：

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以细菌肽聚糖为唯一底物的厌氧富集培养物的微生物群落分析

使用从革兰氏阴性大肠杆菌或革兰氏阳性藤黄微球菌纯化的肽聚糖作为唯一底物，从稻田土壤和厌氧消化池污泥中富集产甲烷微生物群落。甲烷生产数据表明肽聚糖的厌氧降解以及来自大肠杆菌的肽聚糖具有较低的降解性。用大肠杆菌或藤黄体的肽聚糖喂养的富集培养物的群落结构相似，但明显不同。许多系统发育新的和未培养的细菌，特别是在拟杆菌门、WWE1、Armatimonadetes 和疣微菌门中，在富集培养物中占主导地位，表明它们参与了厌氧肽聚糖降解。