AIM To validate a new method for the evaluation of pulp tissue debridement in the root canals of extracted teeth using an impregnation protocol involving potassium triiodide, a radiocontrast solution known as Lugol's, combined with micro-computed tomographic (micro-CT) imaging. METHODOLOGY The impact of NaOCl on the radiopacity of Lugol's solution was assessed using a series of 1:1 dilutions of Lugol in distilled water and 5.25% NaOCl, which were then pipetted into transparent dishes and radiographed. To verify the influence of Lugol on the oxidizing ability of NaOCl, a dissolution test was performed using fresh bovine meat. Ten slices did not undergo any tissue processing, whilst twenty slices were fixed in formaldehyde for 24 h. After that, 10 of them were immersed in Lugol for another 24 h. Then, all specimens were placed in NaOCl and the time required for a complete pulp tissue dissolution was recorded. For the last experiments (histological validation and micro-CT assessment), 8 extracted mandibular premolars with vital pulps were immersed in buffered formalin, scanned in a micro-CT device, accessed, immersed in Lugol for 7 days and scanned again. Then, the root canals of 5 teeth were prepared, scanned, and the volume of remaining pulp tissue identified and quantified, whilst 3 teeth were histologically processed. The same specimens were subjected to histological assessment and the images of the histologic sections were registered with the corresponding micro-CT images to qualitatively verify whether the pulp tissue in the histological sections matched its counterpart in the Lugol-impregnated tissues identified in the micro-CT slices. RESULTS There was no discernible effect on radiopacity when NaOCl was mixed with Lugol. Tissue processing did not affect the time required for the complete dissolution of fresh bovine meat. Histological evaluation revealed a correlation between micro-CT and histological images confirming the identification of Lugol-impregnated pulp tissue in micro-CT images. CONCLUSIONS The radiocontrast Lugol's solution was unaffected by NaOCl and did not interfere with its pulp tissue dissolution capability. The pulp tissue impregnation protocol using Lugol allowed the visualization of pulp tissue on micro-CT images and the identification of remaining pulp tissues during the evaluation of chemical-mechanical canal procedures.

中文翻译：

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对比增强的微型CT评估拔牙后牙髓组织清创术的根治：一系列方法验证的级联实验。

目的通过使用三碘化钾（一种称为Lugol's的放射性对比溶液）的浸渍方案，结合微计算机断层成像（micro-CT）成像，验证一种评估拔牙牙髓组织清创的新方法。方法学使用一系列在蒸馏水中的Lugol 1：1稀释液和5.25％NaOCl评估NaOCl对Lugol溶液的射线不透性的影响，然后将其吸移到透明皿中并进行射线照相。为了验证Lugol对NaOCl氧化能力的影响，使用新鲜的牛肉进行了溶出度测试。十片未经过任何组织处理，而二十片在甲醛中固定了24小时。之后，将其中的10个浸入Lugol中24小时。然后，将所有标本放置在NaOCl中，记录纸浆组织完全溶解所需的时间。对于最后的实验（组织学验证和微CT评估），将8个提取的下颌前磨牙与活髓浸入缓冲的福尔马林中，在微CT装置中扫描，取出，浸入Lugol中7天，然后再次扫描。然后，准备，扫描5颗牙齿的根管，并确定和量化剩余牙髓组织的体积，同时对3颗牙齿进行组织学处理。对相同的标本进行组织学评估，并将组织学切片的图像与相应的Micro-CT图像进行配准，以定性验证组织学切片中的牙髓组织是否与微CT中鉴定的经Lugol浸渍的组织中的对应组织相匹配片。结果当NaOCl与Lugol混合时，对射线不透性没有明显影响。组织处理并不影响新鲜牛肉完全溶解所需的时间。组织学评估揭示了微CT和组织学图像之间的相关性，从而证实了微CT图像中经Lugol浸渍的牙髓组织的鉴定。结论放射性对比剂Lugol溶液不受NaOCl的影响，并且不干扰其牙髓组织的溶解能力。使用Lugol的牙髓组织浸渍方案允许在微CT图像上可视化牙髓组织，并在评估化学机械通道程序的过程中识别剩余的牙髓组织。组织处理并不影响新鲜牛肉完全溶解所需的时间。组织学评估揭示了微CT和组织学图像之间的相关性，从而证实了微CT图像中经Lugol浸渍的牙髓组织的鉴定。结论放射性对比剂Lugol溶液不受NaOCl的影响，并且不干扰其牙髓组织的溶解能力。使用Lugol的牙髓组织浸渍方案允许在微CT图像上可视化牙髓组织，并在评估化学机械通道程序的过程中识别剩余的牙髓组织。组织处理并不影响新鲜牛肉完全溶解所需的时间。组织学评估揭示了微CT和组织学图像之间的相关性，从而证实了微CT图像中经Lugol浸渍的牙髓组织的鉴定。结论放射性对比剂Lugol溶液不受NaOCl的影响，并且不干扰其牙髓组织的溶解能力。使用Lugol的牙髓组织浸渍方案允许在微CT图像上可视化牙髓组织，并在评估化学机械通道程序的过程中识别剩余的牙髓组织。组织学评估揭示了微CT和组织学图像之间的相关性，从而证实了微CT图像中经Lugol浸渍的牙髓组织的鉴定。结论放射性对比剂Lugol溶液不受NaOCl的影响，并且不干扰其牙髓组织的溶解能力。使用Lugol的牙髓组织浸渍方案允许在微CT图像上可视化牙髓组织，并在评估化学机械通道程序的过程中识别剩余的牙髓组织。组织学评估揭示了微CT和组织学图像之间的相关性，从而证实了微CT图像中经Lugol浸渍的牙髓组织的鉴定。结论放射性对比剂Lugol溶液不受NaOCl的影响，并且不干扰其牙髓组织的溶解能力。使用Lugol的牙髓组织浸渍方案允许在微CT图像上可视化牙髓组织，并在评估化学机械通道程序的过程中识别剩余的牙髓组织。