Macroautophagy (autophagy) targets cytoplasmic cargoes to the lysosome for degradation. Like all vesicle trafficking, autophagy relies on phosphoinositide identity, concentration, and localization to execute multiple steps in this catabolic process. Here, we screen for phosphoinositide phosphatases that influence autophagy in Drosophila and identify CG3530. CG3530 is homologous to the human MTMR6 subfamily of myotubularin-related 3-phosphatases, and therefore, we named it dMtmr6. dMtmr6, which is required for development and viability in Drosophila, functions as a regulator of autophagic flux in multiple Drosophila cell types. The MTMR6 family member MTMR8 has a similar function in autophagy of higher animal cells. Decreased dMtmr6 and MTMR8 function results in autophagic vesicle accumulation and influences endolysosomal homeostasis.

中文翻译：

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保守的肌管蛋白相关磷酸酶通过维持自噬通量来调节自噬

巨自噬（自噬）将细胞质货物靶向溶酶体进行降解。与所有囊泡运输一样，自噬依赖于磷酸肌醇的特性、浓度和定位来执行该分解代谢过程中的多个步骤。在这里，我们筛选影响果蝇自噬的磷酸肌醇磷酸酶并鉴定出 CG3530。CG3530 与肌管蛋白相关 3-磷酸酶的人类 MTMR6 亚家族同源，因此，我们将其命名为 dMtmr6。dMtmr6 是果蝇发育和生存所需的，在多种果蝇细胞类型中充当自噬流的调节剂。MTMR6家族成员MTMR8在高等动物细胞的自噬中具有类似的功能。dMtmr6 和 MTMR8 功能下降导致自噬囊泡积聚并影响内溶酶体稳态。