Liver sinusoidal endothelial cells (LSECs) play a key role in the initiation and neoangiogenesis of liver regeneration. We presume that the abnormity of the VEGF/VEGFR2 and its pathway gene Id1, Wnt2 and HGF expression in aged LSECs may be an important mechanism to affect liver regeneration of the elderly. LSECs from two different groups (adult and old) were isolated in a rodent model, and observed by SEM and TEM. The adult and old rats were underwent 70% partial hepatectomy. The proliferation of hepatocytes and LSECs were analyzed by Immunofluorescence staining. The expression of VEGF/VEGFR2 and its pathway gene in isolated LSECs and liver tissue after hepatectomy were detected by qRT-PCR and Western blot. There is a decreased number of endothelial fenestrae in the LSECs of the old group, compared to the adult group. The old group had a lower expression of VEGF/VEGFR2 and its pathway gene than the adult groups (p < 0.01). The results of western blot were consistent with those of qRT-PCR. The hepatocytes had a high proliferation rate at first 4 days after hepatectomy, and a significantly higher proliferation rate in the adult group. The LSECs began to proliferate after 4 days of hepatectomy, and showed a quantity advantage in the adult group. The adult group had a significantly higher expression of VEGF/VEGFR2 and its pathway gene after hepatectomy than the old group (p < 0.01). LSCEs turn to be defenestration in structure and have a low expression of VEGF/VEGFR2 and its pathway gene with aging.

中文翻译：

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衰老对大鼠肝窦内皮细胞VEGF/VEGFR2信号通路基因表达的影响

肝窦内皮细胞 (LSEC) 在肝再生的启动和新血管生成中起关键作用。我们推测老年LSECs中VEGF/VEGFR2及其通路基因Id1、Wnt2和HGF的表达异常可能是影响老年人肝再生的重要机制。在啮齿动物模型中分离来自两个不同组（成人和老年）的 LSEC，并通过 SEM 和 TEM 进行观察。成年和老年大鼠进行了70%的部分肝切除术。通过免疫荧光染色分析肝细胞和LSECs的增殖。通过qRT-PCR和Western blot检测VEGF/VEGFR2及其通路基因在肝切除后分离的LSECs和肝组织中的表达。与成人组相比，老年组的 LSEC 中的内皮窗孔数量减少。老年组VEGF/VEGFR2及其通路基因的表达低于成人组（p < 0.01）。Western Blot结果与qRT-PCR结果一致。肝切除术后第4天肝细胞增殖率高，成人组增殖率明显升高。LSECs在肝切除4天后开始增殖，并在成人组中表现出数量优势。成人组肝切除术后VEGF/VEGFR2及其通路基因表达明显高于老年组（p < 0.01）。LSCEs在结构上表现为开窗，随着年龄的增长，VEGF/VEGFR2及其通路基因的表达降低。肝切除术后第4天肝细胞增殖率高，成人组增殖率明显升高。LSECs 在肝切除 4 天后开始增殖，并在成人组中显示出数量优势。成人组肝切除术后VEGF/VEGFR2及其通路基因表达明显高于老年组（p < 0.01）。LSCEs在结构上表现为开窗，随着年龄的增长，VEGF/VEGFR2及其通路基因的表达降低。肝切除术后第4天肝细胞增殖率高，成人组增殖率明显升高。LSECs在肝切除4天后开始增殖，并在成人组中表现出数量优势。成人组肝切除术后VEGF/VEGFR2及其通路基因表达明显高于老年组（p < 0.01）。LSCEs在结构上表现为开窗，随着年龄的增长，VEGF/VEGFR2及其通路基因的表达降低。成人组肝切除术后VEGF/VEGFR2及其通路基因表达明显高于老年组（p < 0.01）。LSCEs在结构上表现为开窗，随着年龄的增长，VEGF/VEGFR2及其通路基因的表达降低。成人组肝切除术后VEGF/VEGFR2及其通路基因表达明显高于老年组（p < 0.01）。LSCEs在结构上表现为开窗，随着年龄的增长，VEGF/VEGFR2及其通路基因的表达降低。