Introduction. Staphylococcus aureus is a skin and mucous commensal bacterium of warm-blooded animals. In humans, the nose is the main ecological niche of S. aureus , and nasal carriage is a risk factor for developing an endogenous infection. S. aureus nasal colonization is a multifactorial process, involving inter-species interactions among the nasal microbiota. Aims. The objectives of this study were to characterize the microbiota of carriers and non-carriers of S. aureus and to demonstrate the importance of inter-species relationships in the adhesion of S. aureus , a key step in nasal colonization. Methodology. First, we characterized the nasal microbiota from 30 S. aureus carriers and non-carriers by a culturomic approach. We then evaluated the adhesion of S. aureus , first alone and then along with other bacteria of the nasal microbiota. To do that, we used an in vitro model to measure the interactions among bacteria in the presence of epithelial cells. Results. Analysis of the nasal microbiota of the carriers and non-carriers of S. aureus made it possible to observe that each microbiota has specific features in terms of composition. However, this composition differs significantly between carriers and non-carriers mainly through two bacterial groups: coagulase-negative staphylococci and corynebacteria. In a second part, adhesion of S. aureus to epithelial cells showed competition between S. aureus and these bacteria, suggesting a limitation of nasal colonization by S. aureus . Conclusion. These findings demonstrate the existence of a negative correlation between S. aureus and other species which inhibits adhesion and could limit nasal colonization.

中文翻译：

---

金黄色葡萄球菌对上皮细胞的粘附性：一种研究鼻腔菌群内相互作用的体外方法。

介绍。 金黄色葡萄球菌是温血动物的皮肤和粘液共生细菌。在人类中，鼻子是金黄色葡萄球菌的主要生态位，而鼻腔运输是发展内源性感染的危险因素。金黄色葡萄球菌鼻定植是一个多因素过程，涉及鼻微生物群之间的种间相互作用。目的 这项研究的目的是表征金黄色葡萄球菌携带者和非携带者的微生物群，并证明种间关系在金黄色葡萄球菌黏附中的重要性，这是鼻内定殖的关键步骤。方法。 首先，我们通过文化学方法对来自30个金黄色葡萄球菌携带者和非携带者的鼻腔菌群进行了表征。然后，我们首先评估了金黄色葡萄球菌的粘附力，然后评估了鼻微生物群的其他细菌。为此，我们使用了体外模型来测量存在上皮细胞的细菌之间的相互作用。结果。金黄色葡萄球菌携带者和非携带者的鼻腔菌群分析 使人们有可能观察到每种微生物在组成方面都具有特定的特征。但是，这种成分在载体和非载体之间的区别很大，主要是通过两个细菌组：凝固酶阴性葡萄球菌和棒状杆菌。在第二部分中，粘附的金黄色葡萄球菌对上皮细胞显示之间的竞争的金黄色葡萄球菌和这些细菌，这表明通过鼻定植的限制金黄色葡萄球菌。结论。这些发现表明，金黄色葡萄球菌与其他物种之间存在负相关性，该相关性抑制粘附并可能限制鼻部定植。