An anaerobic bacterium Clostridium buturicum QXYZ514 was isolated from a pond soil sample located neighboring to a biodiesel factory. This bacterium possesses excellent metabolic features for converting biodiesel-derived glycerol into various bioproducts, including 1,3-propanediol, butyrate, lactate, and acetate, and fuels, like ethanol and butanol. To further improve the yield of the target products and minimize the production of the by-products, the whole genome sequence of this multipurpose strain might provide necessary genetic information, and hence, the complete genome of QXYZ514 strain was sequenced using the PacBio RS II sequencing method. According to the complete genome sequence, the genome of QXYZ514 consisted of two circular chromosomes with a total of 4,636,461 bp, where GC content was found to be 28.76%. Major predicted features of the genome included a total of 4220 coding sequences (CDS), 87 tRNAs genes, and 36 rRNAs genes, which were annotated with the help of different databases for a better understanding of this strain. Six possible CRISPR components were also predicted in the genome. The exploration of the complete genome sequence of the QXYZ514 strain would have the potential to enrich the diversity of this species, and to recognize some significant hydrolytic enzymes, which could provide the references for overcoming the bottlenecks in the biorefinery usage of this bacterium in the valorization of biodiesel-derived glycerol.

中文翻译：

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酪酸梭菌 QXYZ514 的基因组序列草案，一种在基于废物的生物精炼厂中将甘油转化为燃料和生物产品的强效细菌

从位于生物柴油工厂附近的池塘土壤样品中分离出厌氧菌 Clostridium buturicum QXYZ514。这种细菌具有出色的代谢特性，可将生物柴油衍生的甘油转化为各种生物产品，包括 1,3-丙二醇、丁酸盐、乳酸盐和乙酸盐，以及燃料，如乙醇和丁醇。为了进一步提高目标产物的产量并最大限度地减少副产物的产生，该多用途菌株的全基因组序列可能提供必要的遗传信息，因此，使用 PacBio RS II 测序对 QXYZ514 菌株的全基因组进行测序方法。根据完整的基因组序列，QXYZ514的基因组由两条环状染色体组成，总长度为4,636,461 bp，其中GC含量为28.76%。基因组的主要预测特征包括总共4220个编码序列（CDS）、87个tRNAs基因和36个rRNAs基因，在不同数据库的帮助下对其进行了注释，以更好地了解该菌株。基因组中还预测了六种可能的 CRISPR 成分。对QXYZ514菌株全基因组序列的探索有可能丰富该菌种的多样性，并识别出一些重要的水解酶，为克服该菌生物炼制利用中的瓶颈提供参考。生物柴油衍生的甘油。基因组中还预测了六种可能的 CRISPR 成分。对QXYZ514菌株全基因组序列的探索有可能丰富该菌种的多样性，并识别出一些重要的水解酶，为克服该菌生物炼制利用中的瓶颈提供参考。生物柴油衍生的甘油。基因组中还预测了六种可能的 CRISPR 成分。对QXYZ514菌株全基因组序列的探索有可能丰富该菌种的多样性，并识别出一些重要的水解酶，为克服该菌生物炼制利用中的瓶颈提供参考。生物柴油衍生的甘油。