Crude glycerol (CG) can be used as a substrate for microbial bioconversion. However, due to presence of many impurities, many microorganisms are unable to utilise this substrate efficiently. The present study is trying to improve CG using as the feedstock of Aspergillus terreus for the production of lovastatin, (+)-geodin, and sulochrin. The CG was pretreated chemically (solvents) and physically (activated carbon (AC) and water softener (WS)) to separate most of the impurities from the CG. For solvent pretreatments, petroleum ether (PE) produced the largest increase of lovastatin (92.8%) when compared to positive control and pure glycerol (PG) and up to 820% when compared to negative control (CG). In contrast, diethyl ether (DE) produced the largest increase in (+)-geodin at 80.81% (versus CG) and 176.23% (versus PG). The largest increase in toluene (Tol) was observed in sulochrin production, at 67.22% (versus CG) and 183.85% (versus PG). For physical pretreatments, the pattern of metabolite production in AC (lovastatin: 20.65 mg/L, (+)-geodin: 7.42 mg/L, sulochrin: 11.74 mg/L) resembled PG (lovastatin: 21.8 mg/L, (+)-geodin: 8.60 mg/L, sulochrin: 8.18 mg/L), while WS (lovastatin: 11.25 mg/L, (+)-geodin: 15.38 mg/L, sulochrin: 16.85 mg/L) resembled CG (lovastatin: 7.1 mg/L, (+)-geodin: 17.10 mg/L, sulochrin: 14.78 mg/L) at day 6 of fermentation. These results indicate that solvent pretreatments on CG are excellent for metabolites production in A. terreus, depending on the solvents used. In contrast, physical pretreatments are only feasible for (+)-geodin and sulochrin production. Therefore, different strategies can be employed to manipulate the A. terreus bioconversion using improved CG by using a few simple pretreatment strategies.

中文翻译：

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改善土壤曲霉曲霉粗甘油利用和代谢产物生产的预处理策略

粗甘油（CG）可用作微生物生物转化的底物。然而，由于存在许多杂质，许多微生物不能有效地利用该底物。本研究正在尝试使用曲霉曲霉作为原料来改善CG用于生产洛伐他汀，（+）-geodin和硫磺菊酯。CG经过化学（溶剂）和物理（活性炭（AC）和水软化剂（WS））预处理，以从CG中分离出大多数杂质。对于溶剂预处理，与阳性对照和纯甘油（PG）相比，石油醚（PE）产生的洛伐他汀的增幅最大（92.8％），与阴性对照（CG）相比，增幅高达820％。相反，乙醚（DE）产生的（+）-geodin增幅最大，分别为80.81％（相对于CG）和176.23％（相对于PG）。磺胺菊酯生产中观察到的甲苯（Tol）增幅最大，分别为67.22％（相对于CG）和183.85％（相对于PG）。对于物理预处理，AC（洛伐他汀：20.65 mg / L，（+）-geodin：7.42 mg / L，硫磺菊酯：11.74 mg / L）在AC中的代谢产物生成方式类似于PG（lovastatin：21.8 mg / L，（+）-geodin：8.60 mg / L，磺胺菊酯：8.18 mg / L），而WS（洛伐他汀：11.25 mg / L，（+）-geodin：15.38 mg / L，磺胺菊酯：16.85 mg / L）与CG（洛伐他汀：7.1 mg / L，（+）-大地精：17.10 mg / L，硫磺菊酯：14.78 mg / L）。这些结果表明，CG上的溶剂预处理非常适合代谢产物的产生。土曲霉，取决于使用的溶剂。相反，物理预处理仅适用于（+）-geodin和硫磺菊酯的生产。因此，通过使用一些简单的预处理策略，可以采用不同的策略来利用改良的CG来操纵土壤曲霉的生物转化。