Galega officinalis products have been used for the control of diabetes (type 2) across the world. Experimental and clinical evaluations of galegine substance produced by a medicinal plant (Galega officinalis) provided the pharmacological and chemical basis for metformin discovery which was confirmed for diabetes therapy. In this paper, the molecularly imprinted polymer (MIP) was synthesized for galegine, using galegine as a template molecule, methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, azobisisobutyronitrile (AIBN) as a reaction initiator, and acetonitrile as a solvent. The assisted functional groups, morphology, topographic image of surface, and crystalline structure of synthesized MIP were characterized by FT-IR spectroscopy, field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM) images, and XRD diffraction pattern techniques, respectively. Also, the performance of the mentioned electrode was quantified and qualified by the differential pulse voltammetry technique (DPV). The galegine amount was determined with the polarographic technique. In this research, the galegine extraction conditions were optimized and graphene nanoparticles were used to increase the adsorption. In addition, different parameters affecting extraction were investigated such as MIP adsorbent amount, pH of solution, effect of the surfactant, and ionic compound to achieve high recovery percent. The recovery percent, limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD %) were 4.101 μg·mL−1, 12.427 μg·mL−1, and 1.199% (n = 3), respectively. The results show that the prepared MIP can be used as an effective and inexpensive adsorbent for preconcentration and galegine extraction from a natural sample. It is noteworthy that this developed method was used successfully to determine galegine extracted from Galega officinalis L.

中文翻译：

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氧化石墨烯增强分子印迹聚合物（MIP）技术提取半胱氨酸的新方法及其极谱法评价

Galega药用产品已在世界范围内用于控制糖尿病（2型）。由药用植物（Galega officinalis）产生的半胱氨酸物质的实验和临床评估为二甲双胍的发现提供了药理和化学基础，该二甲双胍被证实可用于糖尿病治疗。本文以半胱氨酸为模板分子，以甲基丙烯酸（MAA）为功能单体，以乙二醇二甲基丙烯酸酯（EGDMA）为交联剂，偶氮二异丁腈（AIBN）为半乳糖苷为模板分子，合成了半乳糖苷分子印迹聚合物（MIP）。反应引发剂和乙腈作为溶剂。通过FT-IR光谱，场发射扫描电子显微镜（FE-SEM）对合成的MIP的辅助官能团，形貌，表面形貌和晶体结构进行了表征。原子力显微镜（AFM）图像和XRD衍射图技术。同样，通过差分脉冲伏安法（DPV）定量和鉴定了上述电极的性能。用极谱法测定半乳精的量。在这项研究中，优化了半胱氨酸的提取条件，并使用了石墨烯纳米颗粒来增加吸附。另外，研究了影响萃取的不同参数，例如MIP吸附剂的量，溶液的pH值，表面活性剂的作用和离子化合物，以实现高回收率。回收率，检出限（LOD），定量限（LOQ）和相对标准偏差（RSD％）为4.101μg·mL-1、12.427μg·mL-1和1.199％（n = 3），分别。结果表明，制备的MIP可以用作从天然样品中进行预浓缩和半胱氨酸提取的有效且廉价的吸附剂。值得注意的是，这种发达的方法已成功用于测定从加利加牛油中提取的半乳糖。