Objective. To investigate the effect of Mn₃O₄ nanoparticles (Mn₃O₄NPs) on inflammatory factors induced by lipopolysaccharide (LPS) in human tendon cells and its mechanism. Methods. The Mn₃O₄NPs were synthesized by a hydrothermal method. RT-qPCR was used to detect the expression levels of miRNAs related to inflammation in human tendon cells. The expression level of NLRP1 (NOD-like receptor containing pyrin domain 1) was measured by Western blotting. ELISA assay was used to measure the level of TNF-α, IL-1β, IL-4, and IL-10. The relationship between miR-181a-5p and NLRP1 was verified by dual-luciferase reporter assay. Results. Mn₃O₄NPs produced in this study were brown spherical particles with an average size of 7-10 nm. Mn₃O₄NP treatment significantly reduced the levels of TNF-α and IL-1β but increased the levels of IL-4 and IL-10 in the human tendon cells induced by LPS. In addition, Mn₃O₄NP treatment remarkably increased the expression level of miR-181a-5p. NLRP1 is one of the targets of miR-181a-5p, and miR-181a-5p downregulated its expression. Further study showed that Mn₃O₄NPs could alleviate the inflammatory response of human tendon cells induced by LPS by upregulating miR-181a-5p and thus downregulating the expression of NLRP1. Conclusion. Mn₃O₄NPs affect the expression of inflammatory cytokines in the human tendon cells induced by LPS by modulating the molecular axis of miR-181a-5p/NLRP1.

中文翻译：

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Mn3O4纳米粒对脂多糖诱导的人肌腱细胞炎性因子的影响及其机制

目标。目的探讨Mn ₃ O ₄纳米颗粒（Mn ₃ O ₄ NPs）对人肌腱细胞中脂多糖（LPS）诱导的炎性因子的影响及其机制。方法。通过水热法合成了Mn ₃ O ₄ NPs。RT-qPCR用于检测与人类肌腱细胞炎症相关的miRNA的表达水平。通过蛋白质印迹法测定NLRP1（含有吡啶结构域1的NOD样受体）的表达水平。ELISA测定用于测量TNF-α的水平α，IL-1 β，IL-4和IL-10。miR-181a-5p和NLRP1之间的关系已通过双萤光素酶报告基因分析验证。结果。这项研究中生产的Mn ₃ O ₄ NP是棕色球形颗粒，平均尺寸为7-10 nm。的Mn ₃ ö ₄ NP治疗显著降低TNF-α的水平α和IL-1 β，但在LPS诱导的人腱细胞增加IL-4和IL-10的水平。此外，Mn ₃ O ₄ NP处理显着提高了miR-181a-5p的表达水平。NLRP1是miR-181a-5p的靶标之一，miR-181a-5p下调了其表达。进一步的研究表明Mn ₃ O_4个NPs通过上调miR-181a-5p从而下调NLRP1的表达来减轻LPS诱导的人肌腱细胞的炎症反应。结论。Mn ₃ O ₄ NP通过调节miR-181a-5p / NLRP1的分子轴来影响LPS诱导的人腱细胞中炎性细胞因子的表达。