Spectroelectrochemical techniques were used to probe the interaction of adenine with pyridoxine at pH 7.0. Analysis of UV-visible absorption of the adenine-pyridoxine complex at 260 nm using the Lineweaver–Burk double reciprocal plot produced a linear graph indicating a 1 : 1 mode of interaction between the compounds and a binding constant of 29.1. Change in the background current and broadening of adenine and pyridoxine cyclic voltammetry (CV) oxidation peaks at 1.0 V and 0.8 V, respectively, compared to the CV of the individual compounds is indicative of an interaction. The Raman shift of the coupled –C(11)H₂-OH bending and in-plane N(7)-H mode at 1235 cm⁻¹ to 1215 cm⁻¹ of pyridoxine and the shift to the lower wavenumber of the adenine –N(10)H₂ rocking band from 942 to 906 cm⁻¹ confirm that the adenine exocyclic amino group and its purine nitrogen atom N(7) interacts with pyridoxine O(12) via the formation of hydrogen bonds. Strong enhancement of surface-enhanced Raman spectroscopy (SERS) bands pertaining to adenine and the bathochromic shift of the normal Raman band due to the adenine ring breathing mode observed at 722 cm⁻¹ in the spectrum of adenine, to 732 cm⁻¹ in the SERS spectrum of aqueous adenine-pyridoxine indicates that the complex adsorbs onto the Ag nanoparticle surface with the adenine portion possessing a perpendicular orientation.

中文翻译：

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在生理pH下腺嘌呤与吡rid醇相互作用的光谱电化学研究

使用光谱电化学技术检测pH 7.0下腺嘌呤与吡ido醇的相互作用。使用Lineweaver-Burk双向倒数图分析260 nm处腺嘌呤-吡py醇络合物的紫外可见吸收，得出线性图，表明化合物之间的相互作用为1：1模式，结合常数为29.1。与单个化合物的CV相比，本底电流的变化以及腺嘌呤和吡ido醇循环伏安法（CV）的氧化峰分别在1.0 V和0.8 V处变宽，表明存在相互作用。在吡35醇的1235 cm ^-1至1215 cm ^-1处耦合的–C（11）H ₂ -OH弯曲和面内N（7）-H模式的拉曼位移和向腺嘌呤的低波数的位移– N（10）H ₂从942至906 cm ^-1的摇摆带证实了腺嘌呤环外氨基及其嘌呤氮原子N（7）通过氢键的形成与吡ido醇O（12）相互作用。强增强表面增强拉曼光谱（SERS）属于腺嘌呤和正常拉曼频带的红移由于在722厘米观察到腺嘌呤环呼吸模式谱带的^-1在腺嘌呤的光谱，以732厘米^-1在水性腺嘌呤-吡ox醇的SERS光谱表明，该复合物以腺嘌呤部分具有垂直取向的方式吸附到Ag纳米颗粒表面上。