Background: Intestinal flora dynamically affects the host's systemic immune system. Liver is one of the organs that may be affected by intestinal microbiota.

Materials and Methods: In this study, we aimed to identify proteome level differences between liver tissue from mice cleared intestinal flora and control using tandem mass spectrometry (LC-MS/MS) and label free quantification. Additionally, protein-protein interactions were mapped by STRING, and also, the enrichment of inflammation-related signaling pathways and biological processes was identified using GO and IPA network system. RT-PCR and Western blot were used for validation of the proteomics findings.

Results: Our study demonstrated that mice with cleared intestinal flora exhibited decreased sensitivity to Concanavalin A induced acute hepatitis. 324 Proteins in liver were differently expressed after intestinal flora clearance for one week while 210 proteins were differently expressed after intestinal flora clearance for two weeks. Furthermore, five of the identified proteins were validated by western blotting and further investigated by semi-quantitative RT-PCR.

Conclusion: Our results showed that intestinal flora clearance in mice could reduce sensitivity to Concanavalin A induced liver injury and influence the expression of proteins in liver, which provides a clue for studying the relationship between gut bacteria and Concanavalin A induced hepatitis.

背景：肠道菌群会动态影响宿主的全身免疫系统。肝脏是可能受肠道菌群影响的器官之一。

材料和方法：在这项研究中，我们旨在鉴定使用串联质谱（LC-MS / MS）和无标记定量分析的肠道菌群清除小鼠和对照小鼠肝脏组织之间蛋白质组水平的差异。此外，通过STRING绘制了蛋白质-蛋白质相互作用的图谱，并使用GO和IPA网络系统鉴定了炎症相关信号通路和生物过程的富集。RT-PCR和蛋白质印迹用于验证蛋白质组学发现。

结果：我们的研究表明，肠道菌群清除的小鼠对伴刀豆球蛋白A诱发的急性肝炎的敏感性降低。肠道菌群清除1周后肝脏中的324种蛋白质表达不同，而肠道菌群清除2周后肝脏中的324种蛋白质表达不同。此外，通过蛋白质印迹验证了其中5种已鉴定的蛋白质，并通过半定量RT-PCR进行了进一步研究。

结论：我们的研究结果表明，小鼠肠道菌群清除可降低对刀豆蛋白A诱导的肝损伤的敏感性并影响肝脏中蛋白质的表达，这为研究肠道细菌与刀豆蛋白A诱导的肝炎之间的关系提供了线索。