Background: Desheng pills (DSP) consist of six traditional Chinese medicine. This preparation is used fornourishing blood, eliminating stasis, soothing liver and regulating menstruation, and can also be used to treat menoxenia and dysmenorrhea caus ed by qi stagnation and blood stasis.

Objective: In this paper, an accurate and sensitive high-performance liquid chromatography-diode array detector (HPLC-DAD) method was developed and validated for simultaneous determination of seven active components (gallic acid, paeoniflorin, costunolide, dehydrocostuslactone, rutin, leonurine hydrochloride and ferulic acid) in the traditional Chinese formula-Desheng pills.

Methods: The seven analytes were separated on Agilent ZORBAX SB-C18 column (250mm× 4.6mm, 5μm) maintained at the temperature of 30. Gradient elution was performed with the mobile phase of methanol (A)-0.1% phosphoric acid solution (B) at the flow rate of 1.0mL·min-1. The analysis was carried out at the wavelength of 225 nm, 256 nm, 277 nm and 320 nm with an injection volume of 10 μL.

Results: The measured seven components showed good linear relationships within their own concentration ranges along with coefficients of determination ≥0.9996. The limits of detection and quantitation of all analytes were in the range of 0.19-13.51 μg/mL and 0.59-40.93 μg/mL, respectively. Average recoveries ranged from 98.82% to 102.01% with RSDs of 1.47%-1.99%. The content of tested components was in the range of 0.053-0.421 mg/g.

Conclusion: The proposed method was found to be sensitive, accurate and reproducible, which provided an effective quantitative analytical method for quality control of Desheng pills.

背景：德胜丸（DSP）由六种中药组成。该制剂用于补血，祛瘀，润肝，调经，也可用于治疗气滞血瘀引起的月经不调和痛经。

目的：本文建立了一种准确灵敏的高效液相色谱-二极管阵列检测器（HPLC-DAD）方法，并验证了该方法可同时测定7种活性成分（没食子酸，flor药苷，木香内酯，脱氢木糖内酯，芦丁，盐酸去草精和阿魏酸）

方法：将七种分析物在温度保持在30℃的Agilent ZORBAX SB-C18色谱柱（250mm×4.6mm，5μm）上分离。用甲醇（A）-0.1％磷酸溶液（B）的流动相以1.0mL·min-1的流速进行梯度洗脱。在225 nm，256 nm，277 nm和320 nm的波长下进行分析，进样量为10μL。

结果：所测得的七个成分在其各自的浓度范围内均具有良好的线性关系，并且测定系数≥0.9996。所有分析物的检出限和定量限分别在0.19-13.51μg/ mL和0.59-40.93μg/ mL范围内。平均回收率从98.82％到102.01％，RSD为1.47％-1.99％。被测成分的含量为0.053-0.421mg / g。

结论：该方法灵敏，准确，可重现，为德胜丸的质量控制提供了一种有效的定量分析方法。