Background: Biosynthesis of gold nanoparticles from medicinal plants has become an interesting strategy in biomedical research due to its exclusive properties including less toxic cellular level through its ecofriendly biological function.

Objective: To examine the anti-lipid accumulation effect of spherical gold nanoparticles (size 10-20 nm) synthesized from Dendropanax morbifera Léveille (D-AuNPs) in both 3T3-L1 and HepG2 cells.

Methods: 3T3-L1 preadipocytes and HepG2 hepatocytes were stimulated with cocktail media to generate obese and fatty liver disease models. Cell cytotoxicity and cell proliferation assays were performed in adipocytes at different stages of growth. An anti-lipid accumulation assay was performed in 3T3-L1 obese and HepG2 fatty liver models using different doses of D-AuNPs. Expression of adipogenic genes of PPARγ, CEBPα, Jak2, STAT3, and ap2 and hepatogenic genes PPARα, FAS, and ACC was measured by real-time PCR. In addition, protein expression of PPARγ and CEBPα was evaluated by immunoblotting assay.

Results: We found that D-AuNPs (size 10–20 nm) at concentrations up to 100 µg/ml were nontoxic to 3T3-L1 and HepG2 at post-confluent and mature stages. In addition, pretreatment of D-AuNPs at post-confluent stage reduced triglyceride content. In addition, the adipogenesis process was negatively controlled by D-AuNPs, with downregulated PPARγ, CEBPα, Jak2, STAT3, and ap2 expression in 3T3-L1 cells and FAS and ACC levels in HepG2 cells.

Conclusion: These data indicated that D-AuNPs exert antiadipogenic properties. We hypothesize that Dendropanax contains a large amount of phenolic compound that coats the surface of gold nanoparticles and has the ability to reduce the excess amount of lipid in both cell lines.

背景：由于其独特的特性（包括通过其生态友好的生物功能降低毒性细胞水平），从药用植物生物合成金纳米颗粒已成为生物医学研究中一种有趣的策略。

目的：研究由Dendropanax morbiferaLéveille（D-AuNPs）合成的球形金纳米颗粒（尺寸为10-20 nm）在3T3-L1和HepG2细胞中的抗脂质蓄积作用。

方法：用鸡尾酒培养基刺激3T3-L1前脂肪细胞和HepG2肝细胞，建立肥胖和脂肪肝疾病模型。在脂肪细胞的不同生长阶段进行细胞毒性和细胞增殖试验。使用不同剂量的D-AuNP在3T3-L1肥胖和HepG2脂肪肝模型中进行了抗脂质蓄积测定。通过实时PCR测量PPARγ，CEBPα，Jak2，STAT3和ap2的成脂基因的表达以及肝成因基因PPARα，FAS和ACC的表达。另外，通过免疫印迹测定评价PPARγ和CEBPα的蛋白质表达。

结果：我们发现，在融合后和成熟阶段，浓度高达100 µg / ml的D-AuNPs（尺寸10–20 nm）对3T3-L1和HepG2无毒。此外，在汇合后阶段对D-AuNPs进行预处理可降低甘油三酯含量。此外，成脂过程受到D-AuNPs的负调控，PPARγ，CEBPα，Jak2，STAT3和ap2在3T3-L1细胞中的表达下调，而FAS和ACC在HepG2细胞中的表达下调。

结论：这些数据表明D-AuNPs具有抗脂肪形成特性。我们假设Dendropanax包含大量的酚类化合物，可以覆盖金纳米颗粒的表面，并具有减少两种细胞系中过量脂质的能力。