Background: Mass spectrometry is a tool used in analytical chemistry to identify components in a chemical compound and it is of tremendous importance in the field of biology for high throughput analysis of biomolecules, among which protein is of great interest.

Objective: Advancement in proteomics based on mass spectrometry has led the way to quantify multiple protein complexes, and proteins interactions with DNA/RNA or other chemical compounds which is a breakthrough in the field of bioinformatics.

Methods: Many new technologies have been introduced in electrospray ionization (ESI) and Matrixassisted Laser Desorption/Ionization (MALDI) techniques which have enhanced sensitivity, resolution and many other key features for the characterization of proteins.

Results: The advent of ambient mass spectrometry and its different versions like Desorption Electrospray Ionization (DESI), DART and ELDI has brought a huge revolution in proteomics research. Different imaging techniques are also introduced in MS to map proteins and other significant biomolecules. These drastic developments have paved the way to analyze large proteins of >200kDa easily.

Conclusion: Here, we discuss the recent advancement in mass spectrometry, which is of great importance and it could lead us to further deep analysis of the molecules from different perspectives and further advancement in these techniques will enable us to find better ways for prediction of molecules and their behavioral properties.

背景：质谱法是一种用于分析化学中鉴定化合物成分的工具，在生物学领域中对生物分子的高通量分析具有极其重要的意义，其中蛋白质非常受关注。

目的：基于质谱的蛋白质组学研究的发展为定量多种蛋白质复合物以及蛋白质与DNA / RNA或其他化合物的相互作用提供了途径，这是生物信息学领域的一项突破。

方法：在电喷雾电离（ESI）和基质辅助激光解吸/电离（MALDI）技术中引入了许多新技术，这些技术具有增强的灵敏度，分辨率和许多其他表征蛋白质的关键特征。

结果：环境质谱技术及其不同版本（如解吸电喷雾电离（DESI），DART和ELDI）的出现带来了蛋白质组学研究的巨大变革。MS中还引入了不同的成像技术，以绘制蛋白质和其他重要生物分子的图。这些巨大的发展为轻松分析> 200kDa的大型蛋白质铺平了道路。

结论：在这里，我们讨论质谱的最新进展，这一进展非常重要，它可能会导致我们从不同的角度对分子进行进一步的深入分析，而这些技术的进一步发展将使我们能够找到更好的分子预测方法及其行为特征。