Background: Cerebrospinal fluid (CSF) contains pro-growth factors that can affect proliferation, migration and differentiation of Mesenchymal Stem Cells (MSCs).

Objective: This study aimed to isolate MSC like cells from CSF of patients with meningioma and psudotumorcerebri (PTC) and identify differentially expressed proteins in these cells.

Methods: Five patients with newly diagnosed intracranial meningioma and five patients with PTC were recruited in this comparative proteomics study. MSCs were isolated from CSF and validated by mesenchyml and non-mesenchyml fluorochrome antibodies, and flow cytometer analysis. Two- Dimensional Gel Electrophoresis (2-DE) coupled with Mass Spectrometry (MS) was performed to identify differentially expressed proteins.

Results: Microscopic views of the isolated cells as well as flow cytometer analysis were found to be compatible with MSC-like cells. Eight distinct protein spots were differentially and reproducibly expressed among the stained gels of two studied groups. The identified proteins were Phosphoglycerate Mutase 1 (PGAM1), LIM and SH3 domain protein (LASP1), peroxiredoxin-6 (PRDX-6), type I cytoskeletal 9 (KRT9), Superoxide Dismutase (SOD), endoplasmin, Stathmin 1 (STMN1), and glutathione S-transferase (GST).

Conclusion: This study provides new insights into the plausible role of CSF derived MSCs in cancer progression, and reveals a promising therapeutic opportunity for targeting of MSC proteins in patients with meningioma.

背景：脑脊液（CSF）含有促生长因子，可影响间充质干细胞（MSCs）的增殖，迁移和分化。

目的：本研究旨在从脑膜瘤和假性脑瘤（PTC）患者的脑脊液中分离出MSC样细胞，并鉴定这些细胞中差异表达的蛋白质。

方法：这项比较蛋白质组学研究招募了5例新诊断的颅内脑膜瘤患者和5例PTC患者。从CSF中分离出MSC，并通过间充质和非间充质荧光色素抗体和流式细胞仪分析进行验证。进行了二维凝胶电泳（2-DE）与质谱（MS）的耦合，以鉴定差异表达的蛋白质。

结果：发现分离细胞的显微镜观察以及流式细胞仪分析与MSC样细胞相容。在两个研究组的染色凝胶中，八个不同的蛋白质斑点差异且可重复表达。鉴定出的蛋白质为磷酸甘油酸突变酶1（PGAM1），LIM和SH3域蛋白（LASP1），过氧化物酶毒素6（PRDX-6），I型细胞骨架9（KRT9），超氧化物歧化酶（SOD），内质酶，Stathmin 1（STMN1） ，以及谷胱甘肽S-转移酶（GST）。

结论：这项研究为脑脊液来源的MSCs在癌症进展中的合理作用提供了新的见解，并揭示了靶向脑膜瘤患者MSC蛋白的有希望的治疗机会。