Background: Staphylococcus aureus (S. aureus), an important pathogen, causes a wide range of infections in human starting from food poisoning to septicemia. It affects the host cells with various exotoxins, known as virulence factors, which are synthesized in growth phase-dependent manner of the bacteria. S. aureus has been reported to become resistant to antibiotics rapidly. Among two common clinical isolates, Methicillin-sensitive S. aureus (MSSA) and Methicillin-resistant S. aureus (MRSA), MRSA pose major problems across hospitals around the world.

Objective: The objective of the present study was to profile the exoproteins of Methicillin-sensitive S. aureus (ATCC 25293) and subsequently to establish a proteomics-based method of characterization of S. aureus that is crucial in treating hospital-acquired infections.

Methods: We used two-dimensional nanoLC/ESI-MS based proteomic platform to characterize and quantify the exoproteins isolated from Methicillin-sensitive S. aureus (ATCC 25293) strain.

Results: A total of 69 proteins were identified from extracellular proteome pool of ATCC 25293 strain that includes 18 extracellular proteins, 40 cytoplasmic proteins, 2 membrane proteins, 3 cell wall proteins and 6 uncharacterized proteins.

Conclusion: We propose that this mass spectrometry-based proteomics method of characterization of exoproteins might be useful to identify S. aureus strains that are resistant to antibiotics.

中文翻译：

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金黄色葡萄球菌细胞外蛋白质组学分析：基于质谱的蛋白质组学方法的外毒素表征。

背景：金黄色葡萄球菌（S. aureus）是一种重要的病原体，从食物中毒到败血病，都会引起人类广泛的感染。它以各种外毒素（称为毒力因子）影响宿主细胞，这些外毒素以细菌的生长期依赖性方式合成。据报道，金黄色葡萄球菌迅速对抗生素产生抗药性。在两种常见的临床分离株中，对甲氧西林敏感的金黄色葡萄球菌（MSSA）和对耐甲氧西林的金黄色葡萄球菌（MRSA），MRSA在世界各地的医院中都构成了主要问题。

目的：本研究的目的是分析对甲氧西林敏感的金黄色葡萄球菌的外蛋白（ATCC 25293），并随后建立基于蛋白质组学的金黄色葡萄球菌表征方法，该方法对治疗医院获得性感染至关重要。

方法：我们使用基于二维nanoLC / ESI-MS的蛋白质组学平台来表征和定量分离对甲氧西林敏感的金黄色葡萄球菌（ATCC 25293）菌株的外蛋白。

结果：从ATCC 25293菌株的细胞外蛋白质组库中鉴定出总共69种蛋白质，包括18种细胞外蛋白质，40种细胞质蛋白质，2种膜蛋白，3种细胞壁蛋白和6种未表征的蛋白质。

结论：我们建议这种基于质谱的蛋白质组学方法表征外蛋白可能有助于鉴定对抗生素有抗性的金黄色葡萄球菌菌株。