Background: Cervical cancer arises from the cervix and it is the 3rd most diagnosed malignancy and a foremost cause of cancer-related death in females. On the other hand, the expressions of EGFR and p53 are two important proteins observed in various studies on cervical cancer.

Objective: The study aims to evaluate the beneficial effect of radiotherapy based on the regulation of p53 and EGFR gene in patients with cervical cancer.

Methods: In this investigation, the regulation of important molecules responsible for cancer cell proliferation and DNA repair in the cervical cancer cell line was evaluated. The study comprises of an evaluation based on clinical study design from the malignant biopsies of 15 cervical cancer patients. The patterns of expression for the p53 gene and Epidermal Growth Factor Receptor (EGFR) were evaluated in DoTc2 and SiHa cervical cancer cell lines using clonogenic assay, western blotting and immunohistochemistry techniques from the malignant biopsies of the 15 patients.

Results: The study observed that the regulation of p53 and EGFR was very weak after the exposure of the radiation. In addition, the expression of p53 and EGFR was observed in malevolent biopsy samples after radiation with a dosage of 1.8 Gy radiations. Additionally, the expression of p53 and EGFR was able to induce by a single dose of radiotherapy in the malignant biopsies whereas it was unable to induce in DoTc2 and SiHa cervical cancer cells.

Conclusion: The study observed that radiation exposed cancer cell lines modulates the expression of p53 and EGFR gene. The study also highlights the gap between in vitro experimental models and clinical study design.

中文翻译：

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宫颈癌放疗在EGFR和p53基因表达中的作用

背景：子宫颈癌是子宫颈癌，它是第三大被诊断出的恶性肿瘤，也是导致女性癌症相关死亡的首要原因。另一方面，EGFR和p53的表达是在宫颈癌的各种研究中观察到的两个重要蛋白。

目的：研究基于p53和EGFR基因调控的放射治疗对子宫颈癌患者的疗效。

方法：在这项研究中，评估了子宫颈癌细胞系中负责癌细胞增殖和DNA修复的重要分子的调控。该研究包括一项基于临床研究设计的评估，该评估来自15位子宫颈癌患者的恶性活检。使用克隆发生测定，Western印迹和免疫组化技术对15例患者的DoTc2和SiHa宫颈癌细胞系中的p53基因和表皮生长因子受体（EGFR）的表达模式进行了评估。

结果：研究发现，暴露于辐射后，p53和EGFR的调节非常弱。另外，在以1.8Gy辐射剂量照射后，在恶性活检样品中观察到p53和EGFR的表达。此外，在恶性活组织检查中，单次放疗就能诱导p53和EGFR的表达，而在DoTc2和SiHa宫颈癌细胞中不能诱导p53和EGFR的表达。

结论：该研究观察到放射线照射的癌细胞系可调节p53和EGFR基因的表达。该研究还强调了体外实验模型与临床研究设计之间的差距。