Lipid-derived electrophile (LDE) modifications, which are covalent modifications of proteins by endogenous LDEs, are essential types of protein posttranslational modifications. LDE modifications alter the protein structure and regulate their biological processes in cells. LDE modifications of proteins are also closely associated with several diseases and function as potential biomarkers for clinical diagnosis. The crucial step in studying the LDE modifications is to enrich the LDE modified proteins/peptides from complex biological samples with high efficiency and high selectivity and quantify modified proteins/peptides with high accuracy. In this review, we summarize the recent progress in MS-based proteomic technologies to globally identify and quantify LDE modified proteomes, mainly focusing on discussing the qualitative and quantitative technologies.

中文翻译：

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通过质谱法对脂质衍生的亲电修饰蛋白质组进行定性和定量分析的样品制备方法

脂质衍生亲电试剂 (LDE) 修饰是内源性 LDE 对蛋白质的共价修饰，是蛋白质翻译后修饰的基本类型。LDE 修饰改变蛋白质结构并调节它们在细胞中的生物过程。蛋白质的 LDE 修饰也与多种疾病密切相关，并作为临床诊断的潜在生物标志物发挥作用。研究 LDE 修饰的关键步骤是从复杂的生物样品中高效、高选择性地富集 LDE 修饰的蛋白质/肽，并高精度地量化修饰的蛋白质/肽。在这篇综述中，我们总结了基于 MS 的蛋白质组学技术在全局识别和量化 LDE 修饰的蛋白质组方面的最新进展，