The ability to generate programmable vertically aligned 1D nanoscale substrate topography has spurred advances in five fields of cellular nanotechnology: nanoelectrode-based electrophysiology, intracellular delivery, biosensing, mechanotransduction, and – the focus of this review – understanding the key parameters that govern nanostructure-mediated electroporation for diverse cellular manipulations. The integration of 1D nanostructures into conventional cell manipulation and interrogation systems, based on electroporation, has engendered significant interest over the past decade. We evaluate the latest and most influential studies on engineered nanostructure-mediated electroporation platforms, focusing on the use of tuneable, vertically configured nanostructures – in particular, vertically aligned nanowire, nanostraw, and nanotube arrays – to orchestrate cellular processes such as intracellular delivery, biomolecular extraction, and action potential probing, via both experimental and theoretical studies.

中文翻译：

---

垂直配置的纳米结构介导的电穿孔：细胞内调控和讯问的有前途的途径

生成可编程的垂直对齐的一维纳米尺度衬底形貌的能力推动了细胞纳米技术的五个领域的进步：基于纳米电极的电生理学，细胞内传递，生物传感，机械转导，并且-这篇综述的重点-了解控制纳米结构介导的关键参数电穿孔可用于多种细胞操作。在过去的十年中，将一维纳米结构整合到基于电穿孔的常规细胞操作和询问系统中引起了极大的兴趣。我们评估有关工程化纳米结构介导的电穿孔平台的最新，最有影响力的研究，重点是可调谐，垂直配置的纳米结构的使用，尤其是垂直排列的纳米线，纳米吸管，通过实验和理论研究。