Introduction:

This study aims at probing into the expression and biological function of long non-coding RNA (lncRNA) TMPO-AS1 in non-small cell lung cancer (NSCLC), and exploring its regulatory role for miR-204-3p and erb-b2 receptor tyrosine kinase 2 (ERBB2).

Methods:

In this study, paired NSCLC samples were collected, and the expression levels of TMPO-AS1, miR-204-3p and ERBB2 were examined by quantitative real-time polymerase chain reaction (qRT-PCR); proliferative ability and colony formation ability were detected by CCK-8 assay and plate colony formation assay, respectively; flow cytometry was performed to detect the effect of TMPO-AS1 on apoptosis; Transwell assay was used to detect the changes of migration and invasion; qRT-PCR and Western blot were utilised to analyse the changes of miR-204-3p and ERBB2 regulated by TMPO-AS1; luciferase reporter gene assay and RNA immunoprecipitation assay were employed to determine the regulatory relationship between TMPO-AS1 and miR-204-3p.

Results:

We demonstrated that TMPO-AS1 was significantly up-regulated in cancerous tissues of NSCLC samples, and positively correlated with the expression of ERBB2, while negatively correlated with miR-204-3p. After transfection of TMPO-AS1 shRNAs into NSCLC cells, the malignant phenotypes of NSCLC cells were significantly inhibited, while overexpression of TMPO-AS1 had opposite effects; TMPO-AS1 was also demonstrated to regulate the expression of miR-204-3p by sponging it, and indirectly modulate the expression of ERBB2.

Conclusion:

Collectively, we conclude that TMPO-AS1 has the potential to be the ‘ceRNA’ to regulate the expression of ERBB2 by sponging miR-204-3p in NSCLC.

中文翻译：

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LncRNA TMPO-AS1 通过海绵化 miR-204-3p 上调 ERBB2 促进 NSCLC 细胞的增殖和转移。

介绍：

本研究旨在探讨长链非编码RNA（lncRNA）TMPO-AS1在非小细胞肺癌（NSCLC）中的表达及生物学功能，探讨其对miR-204-3p和erb-b2受体的调控作用。酪氨酸激酶 2 (ERBB2)。

方法：

本研究收集配对的NSCLC样本，采用实时定量聚合酶链反应（qRT-PCR）检测TMPO-AS1、miR-204-3p和ERBB2的表达水平；CCK-8法和平板集落形成法分别检测增殖能力和集落形成能力；流式细胞仪检测TMPO-AS1对细胞凋亡的影响；Transwell法检测迁移和侵袭的变化；采用qRT-PCR和Western blot分析TMPO-AS1调控的miR-204-3p和ERBB2的变化；荧光素酶报告基因测定和RNA免疫沉淀测定用于确定TMPO-AS1和miR-204-3p之间的调节关系。

结果：

我们证明 TMPO-AS1 在 NSCLC 样本的癌组织中显着上调，并且与 ERBB2 的表达呈正相关，而与 miR-204-3p 呈负相关。TMPO-AS1 shRNAs转染NSCLC细胞后，NSCLC细胞的恶性表型得到明显抑制，而过表达TMPO-AS1则相反；TMPO-AS1 也被证明可以通过海绵调节 miR-204-3p 的表达，并间接调节 ERBB2 的表达。

结论：

总之，我们得出结论，TMPO-AS1 有可能成为“ceRNA”，通过在 NSCLC 中形成 miR-204-3p 来调节 ERBB2 的表达。