Operable under ambient light and providing chemical selectivity, stimulated Raman scattering (SRS) microscopy opens a new window for imaging molecular events on a human subject, such as filtration of topical drugs through the skin. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores.

中文翻译：

---

通过远程聚焦受激拉曼散射显微镜进行体内体积化学成像

可在环境光下操作并提供化学选择性，受激拉曼散射 (SRS) 显微镜为人类受试者的分子事件成像打开了一个新窗口，例如通过皮肤过滤局部药物。体积 SRS 成像的典型方法是通过物镜的压电扫描，这通常会干扰样品并提供低轴向扫描速率。为了应对这些挑战，我们开发了一种基于可变形镜的远程聚焦 SRS 显微镜，它不仅可以在没有物镜机械扫描的情况下实现高质量的体积化学成像，而且还可以同时校正系统像差。使用远程聚焦 SRS 显微镜，我们对活细胞进行了体积化学成像，并实时捕获了局部化学物质进入人体汗孔的动态扩散。