Isothermal SARS-CoV-2 diagnostics: Tools for enabling distributed pandemic testing as a means of supporting safe reopenings.,ACS Synthetic Biology

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Isothermal SARS-CoV-2 diagnostics: Tools for enabling distributed pandemic testing as a means of supporting safe reopenings.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-09-23 , DOI: 10.1021/acssynbio.0c00359
Pavana Khan ₁ , Lauren M Aufdembrink ₁ , Aaron E Engelhart ₁

Affiliation

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, poses grave threats to both the global economy and health. The predominant diagnostic screens in use for SARS-CoV-2 detection are molecular techniques such as nucleic acid amplification tests. In this Review, we compare current and emerging isothermal diagnostic methods for COVID-19. We outline the molecular and serological techniques currently being used to detect SARS-CoV-2 infection, past or present, in patients. We also discuss ongoing research on isothermal techniques, CRISPR-mediated detection assays, and point-of-care diagnostics that have potential for use in SARS-CoV-2 detection. Large-scale viral testing during a global pandemic presents unique challenges, chief among them the simultaneous need for testing supplies, durable equipment, and personnel in many regions worldwide, with each of these regions possessing testing needs that vary as the pandemic progresses. The low-cost isothermal technologies described in this Review provide a promising means by which to address these needs and meet the global need for testing of symptomatic individuals as well as provide a possible means for routine testing of asymptomatic individuals, providing a potential means of safely enabling reopenings and early monitoring of outbreaks.

中文翻译：

等温 SARS-CoV-2 诊断：用于实现分布式大流行检测作为支持安全重新开放的手段的工具。

由 SARS-CoV-2 病毒引起的 COVID-19 大流行对全球经济和健康构成严重威胁。用于 SARS-CoV-2 检测的主要诊断筛查是核酸扩增测试等分子技术。在这篇综述中，我们比较了当前和新兴的 COVID-19 等温诊断方法。我们概述了目前用于检测患者过去或现在的 SARS-CoV-2 感染的分子和血清学技术。我们还讨论了正在进行的等温技术、CRISPR 介导的检测分析和具有用于 SARS-CoV-2 检测潜力的护理点诊断的研究。全球大流行期间的大规模病毒检测提出了独特的挑战，其中最主要的是全球许多地区同时需要检测用品、耐用设备和人员，而每个地区的检测需求随着大流行的进展而变化。本综述中描述的低成本等温技术提供了一种有前景的方法来满足这些需求并满足全球对有症状个体进行检测的需求，并为无症状个体的常规检测提供了一种可能的方法，从而提供了一种安全的潜在方法。实现重新开放和早期监测疫情。

更新日期：2020-11-21

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