A cold atmospheric plasma unit was used to deposit a biologic, in this case collagen, onto a surface. A collagen coating was applied to 96-well polystyrene plates at a range of powers to determine the effects of the plasma power on the coating structure and viability. Plasma characterization was carried out using voltage, current, and power measurements. Coating characterization was completed using gravimetric measurement, cell growth, water contact angle, as well as spectroscopic analysis and compared to commercial collagen-coated plates. Cell culture studies were also undertaken. The plasma coating matched the performance of the commercial plate but dramatically reduced production time and cost. This method could allow for automated inline production of collagen-coated plates for cell culture applications.

中文翻译：

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在96孔聚苯乙烯板上进行细胞培养的直接胶原蛋白血浆沉积

使用冷大气等离子体单元将生物制剂（在这种情况下为胶原蛋白）沉积到表面上。将胶原蛋白涂层以一定范围的功率施加到96孔聚苯乙烯板上，以确定血浆功率对涂层结构和活力的影响。使用电压，电流和功率测量进行等离子体表征。使用重量分析，细胞生长，水接触角以及光谱分析完成涂层表征，并与商用胶原涂层板进行比较。还进行了细胞培养研究。等离子涂层与商用板的性能相匹配，但是大大减少了生产时间和成本。这种方法可以自动在线生产用于细胞培养的胶原蛋白包被的板。