A Novel Polydopamine Grafted 3D MOF Nanocubes Mediated GR-5/GC DNAzyme Complex with Enhanced Fluorescence Emission Response toward Spontaneous Detection of Pb(II) and Ag(I) Ions,ACS Omega

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A Novel Polydopamine Grafted 3D MOF Nanocubes Mediated GR-5/GC DNAzyme Complex with Enhanced Fluorescence Emission Response toward Spontaneous Detection of Pb(II) and Ag(I) Ions
ACS Omega ( IF 3.7 ) Pub Date : 2020-09-24 , DOI: 10.1021/acsomega.0c03257
Pavadai Rajaji ₁ , Perumal Panneerselvam ₁

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In this work, we have proposed a novel DNAzyme/MnCoPBAs-PDANCs complex-based fluorescence biosensor for subsequent detection of Pb²⁺ and Ag⁺ ions. The GR-5/GC-rich DNAzymes are strongly anchored or quenched on the surface of polydopamine hybridized 3D metal–organic framework MnCoPBAs-PDANCs by π–π stacking interaction. Addition of Pb²⁺ ions has exhibited a catalytic inner cleavage of DNAzyme complex and disturbs to release shorter GC-rich sequence over the surface of MnCoPBAs-PDANCs complexes. Later on, addition of intercalating dye ThT interacts with free GC-rich substrate strand to form a G-quadruplex-ThT structure and thereby effectively enhanced the fluorescence intensity (“turn-on”). Interestingly, subsequent addition of Ag⁺ ions has an uncoiled GQ-ThT structure to provide a robust double-stranded DNA featuring C–Ag⁺–C, which diminishes (“turn-off”) the fluorescence intensity. This improved hybrid sensor exhibited a linear response in a concentration range of 3–9 nM for Pb²⁺, while 4–20 nM for Ag⁺ ions with a lower detection limit of 1.6 and 4.2 nM, respectively. Further, the method was successfully implemented for the analysis of Pb²⁺ and Ag⁺ ions in real water samples with a good regaining and high efficacy for practical analysis.

中文翻译：

新型的聚多巴胺接枝的3D MOF纳米立方体介导的GR-5 / GC DNAzyme复合物具有增强的对自发检测Pb（II）和Ag（I）离子的荧光发射响应

在这项工作中，我们提出了一种新颖的基于DNAzyme / MnCoPBAs-PDANCs络合物的荧光生物传感器，用于随后检测Pb ²⁺和Ag ⁺离子。富含GR-5 / GC的DNA酶通过π-π堆积相互作用牢固地锚定或淬灭在聚多巴胺杂化的3D金属-有机框架MnCoPBAs-PDANCs的表面上。Pb ²⁺离子的添加已显示了DNAzyme复合物的催化内部裂解，并干扰了在MnCoPBAs-PDANCs复合物表面上释放较短的富含GC的序列。随后，添加嵌入染料ThT与富含GC的游离底物链相互作用，形成G-quadruplex-ThT结构，从而有效地增强了荧光强度（“开启”）。有趣的是，随后添加了Ag ⁺离子具有未卷曲的GQ-ThT结构，可提供具有C–Ag ⁺ –C的坚固双链DNA ，从而减弱（“关闭”）荧光强度。改进后的混合传感器在Pb ²⁺的浓度范围为3–9 nM时表现出线性响应，而在Ag ⁺离子的浓度范围为4–20 nM时，检测限分别为1.6和4.2 nM。此外，该方法已成功实现了对真实水样中Pb ²⁺和Ag ⁺离子的分析，具有良好的重获性和实用性。

更新日期：2020-10-06

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