Circular RNAs (circRNAs) play an important regulatory role in a variety of malignancies. Nevertheless, the role of circ_0000142 in multiple myeloma (MM) and its regulatory mechanism remains largely unknown. Real-time polymerase chain reaction was employed to detect the expressions of circ_0000142 and miR-610 in MM tissues and cell lines. The expression of AKT3 and apoptosis-related proteins (Bcl-2, Bax) in MM cells was detected by western blot. The correlation between the expression level of circ_0000142 and the clinicopathological parameters of MM patients was analysed. Cell proliferation, apoptosis, migration and invasion were monitored by Cell Counting Kit 8 assay, flow cytometry analysis and Transwell assay, respectively. The dual-luciferase reporter gene assay and RNA immunoprecipitation assay were employed to verify the targeting relationship between circ_0000142 and miR-610. In this study, it was demonstrated that, circ_0000142 was highly expressed in MM patients, and its high expression level was significantly associated with increased International Staging System and Durie–Salmon stage. Overexpression of circ_0000142 enhanced MM cell proliferation, migration, invasion and suppressed cell apoptosis, while knocking down circ_0000142 had the opposite effects. Mechanistically, circ_0000142 functioned as a competitive endogenous RNA, directly targeting miR-610 and positively regulating AKT3 expression. In brief, circ_0000142 enhances the proliferation and metastasis of MM cells by modulating the miR-610/AKT3 axis.

中文翻译：

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circ_0000142的上调通过吸附miR-610和上调AKT3的表达促进多发性骨髓瘤的进展

环状RNA（circRNA）在多种恶性肿瘤中起重要的调节作用。尽管如此，circ_0000142在多发性骨髓瘤（MM）中的作用及其调节机制仍然未知。实时聚合酶链反应被用来检测circ_0000142和miR-610在MM组织和细胞系中的表达。Western blot检测AKT3及凋亡相关蛋白（Bcl-2，Bax）在MM细胞中的表达。分析了circ_0000142的表达水平与MM患者的临床病理参数之间的相关性。细胞增殖，凋亡，迁移和侵袭分别通过Cell Counting Kit 8测定，流式细胞术分析和Transwell测定进行监测。采用双荧光素酶报告基因测定和RNA免疫沉淀测定来验证circ_0000142和miR-610之间的靶向关系。在这项研究中，证明circ_0000142在MM患者中高表达，其高表达水平与国际分期系统和Durie-Salmon分期的增加显着相关。circ_0000142的过表达增强了MM细胞的增殖，迁移，侵袭并抑制了细胞凋亡，而敲除circ_0000142具有相反的作用。从机理上讲，circ_0000142充当竞争性内源RNA，直接靶向miR-610并正向调节AKT3表达。简而言之，circ_0000142通过调节miR-610 / AKT3轴来增强MM细胞的增殖和转移。结果表明，circ_0000142在MM患者中高表达，其高表达水平与国际分期系统和Durie-Salmon分期的增加显着相关。circ_0000142的过表达增强了MM细胞的增殖，迁移，侵袭并抑制了细胞凋亡，而敲除circ_0000142具有相反的作用。从机理上讲，circ_0000142充当竞争性内源RNA，直接靶向miR-610并正向调节AKT3表达。简而言之，circ_0000142通过调节miR-610 / AKT3轴来增强MM细胞的增殖和转移。结果表明，circ_0000142在MM患者中高表达，其高表达水平与国际分期系统和Durie-Salmon分期的增加显着相关。circ_0000142的过表达增强了MM细胞的增殖，迁移，侵袭并抑制了细胞凋亡，而敲除circ_0000142具有相反的作用。从机理上讲，circ_0000142充当竞争性内源RNA，直接靶向miR-610并正向调节AKT3表达。简而言之，circ_0000142通过调节miR-610 / AKT3轴来增强MM细胞的增殖和转移。circ_0000142的过表达增强了MM细胞的增殖，迁移，侵袭并抑制了细胞凋亡，而敲除circ_0000142具有相反的作用。从机理上讲，circ_0000142充当竞争性内源RNA，直接靶向miR-610并正向调节AKT3表达。简而言之，circ_0000142通过调节miR-610 / AKT3轴来增强MM细胞的增殖和转移。circ_0000142的过表达增强了MM细胞的增殖，迁移，侵袭并抑制了细胞凋亡，而敲除circ_0000142具有相反的作用。从机理上讲，circ_0000142充当竞争性内源RNA，直接靶向miR-610并正向调节AKT3表达。简而言之，circ_0000142通过调节miR-610 / AKT3轴来增强MM细胞的增殖和转移。