Human milk is a significant source of different CD133⁺ and/or CD34⁺ stem/progenitor-like cell subsets in healthy women but their cell distribution and percentages in this compartment of HIV-positive women have not been explored. To date, a decrease of CD34⁺ hematopoietic stem and progenitor cell frequencies in peripheral blood and bone marrow of HIV-positive patients has been reported. Herein, human milk and peripheral blood samples were collected between day 2–15 post-partum from HIV-positive and HIV-negative women, and cells were stained with stem cell markers and analyzed by flow cytometry. We report that the median percentage of CD45^+/highCD34⁻CD133⁺ cell subset from milk and blood was significantly higher in HIV-positive than in HIV-negative women. The percentage of CD45^dimCD34⁻CD133⁺ cell subset from blood was significantly higher in HIV-positive than HIV-negative women. Moreover, percentages of CD45^dimCD34⁺, CD45^dimCD34⁺CD133⁻, and CD45^+highCD34⁺CD133⁻ cell subsets from blood were significantly lower in HIV-positive than HIV-negative women. The CD133⁺ stem/progenitor-like cell subsets are increased in early human milk and blood of HIV-positive women and are differentially distributed to CD34⁺ cell subset frequencies which are decreased in blood.

人乳是健康女性中不同CD133 ⁺和/或CD34 ⁺干细胞/祖细胞样细胞亚群的重要来源，但尚未研究其在HIV阳性女性中的细胞分布和百分比。迄今为止，已经报道了HIV阳性患者外周血和骨髓中CD34 ⁺造血干细胞和祖细胞频率的减少。在此，在产后2-15天之间从HIV阳性和HIV阴性的妇女中收集人乳和外周血样品，并用干细胞标记物对细胞进行染色并通过流式细胞仪进行分析。我们报告CD45 ^{+ /高}CD34 ⁻ CD133 ⁺的中位数百分比HIV阳性女性的牛奶和血液中的细胞亚群显着高于HIV阴性女性。HIV阳性妇女的血液中CD45^弱CD34 ⁻ CD133 ⁺细胞亚群的百分比显着高于HIV阴性妇女。此外，CD45的百分比^昏暗的CD34 ⁺，CD45^暗淡的CD34 ⁺ CD133 ^-和CD45 ^+高CD34 ⁺ CD133 ^-从血细胞亚群显著比HIV阴性的妇女HIV阳性降低。CD133 ⁺在HIV阳性女性的早期母乳和血液中，干/祖细胞样细胞亚群增加，并以CD34 ⁺细胞亚群的频率差异分布，而血液中CD34 ⁺细胞亚群的频率降低。