The aim of this project was to improve the Aspergillus terreus strain and pretreatment of sugarcane bagasse as carrier substrate for bulk production of lovastatin, a cholesterol-lowering drug, in solid state fermentation. Sugarcane bagasse was treated with alkali (1–3% NaOH) for the conversion of complex polysaccharides into simple sugars for better utilization of carrier substrate by microorganism for maximum lovastatin production. Ethidium bromide (time of exposure 30–180 min) was used to induce mutation in Aspergillus terreus and the best mutant was selected on the basis of inhibition zone appeared on petri plates. Fermented lovastatin was quantified by high-performance liquid chromatography. The fermented lovastatin, produced by parent and mutant Aspergillus terreus strain, was checked on body weight, blood glucose and serum cholesterol, ALT, AST, HDL-C, LDL-C, TG and TC levels of rats for their cholesterol lowering capacity. Our results indicate that selected strain along with 2% NaOH treated sugar cane bagasse was best suitable for bulk production of lovastatin by fermentation and fermented lovastatin effectively lower the cholesterol level of rats.

中文翻译：

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洛伐他汀超生产和体内评价的一锅策略的开发

该项目的目的是改进土曲霉菌株和甘蔗渣的预处理，作为载体底物，在固态发酵中批量生产降胆固醇药物洛伐他汀。甘蔗渣用碱（1-3% NaOH）处理，将复杂的多糖转化为单糖，以便微生物更好地利用载体底物，从而最大限度地生产洛伐他汀。使用溴化乙锭（暴露时间 30-180 分钟）诱导土曲霉突变，并根据培养皿上出现的抑菌圈选择最佳突变体。发酵洛伐他汀通过高效液相色谱法定量。由亲本和突变土曲霉菌株生产的发酵洛伐他汀进行了体重、血糖和血清胆固醇、ALT、AST、HDL-C、LDL-C、TG 和 TC 水平的大鼠降低胆固醇的能力。我们的结果表明，所选菌株与 2% NaOH 处理的甘蔗渣最适合通过发酵批量生产洛伐他汀，发酵洛伐他汀可有效降低大鼠的胆固醇水平。